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验证数据展示

  • WB analysis of human plasma using 14181-1-AP

    WB analysis of human plasma using 14181-1-AP

    human plasma were subjected to SDS PAGE followed by western blot with 14181-1-AP (alpha-1B-glycoprotein antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human hepatocirrhosis using 14181-1-AP

    IHC staining of human hepatocirrhosis using 14181-1-AP

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 14181-1-AP (Alpha 1B-Glycoprotein antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human hepatocirrhosis using 14181-1-AP

    IHC staining of human hepatocirrhosis using 14181-1-AP

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 14181-1-AP (Alpha 1B-Glycoprotein antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 14181-1-AP

    IF Staining of HepG2 using 14181-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 14181-1-AP (alpha-1B-glycoprotein antibody) at dilution of 1:25 and Alexa Fluor 488-Conjugated Goat Anti-Rabbit IgG(H+L).

Alpha 1B-Glycoprotein Polyclonal antibody

Alpha 1B-Glycoprotein Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 14181-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, ELISA

别名

A1BG, A1B, Alpha-1-B glycoprotein, Alpha 1B glycoprotein, alpha 1 B glycoprotein

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inhuman plasma tissue, BxPC-3 cells, MCF-7 cells, mouse kidney tissue
Positive IHC detected inhuman hepatocirrhosis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:20000
Immunohistochemistry (IHC)IHC : 1:2500-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14181-1-AP targets Alpha 1B-Glycoprotein in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag5414

Product name: Recombinant human A1BG protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-373 aa of BC035719

Sequence: MAPVSWITPGLKTTAVCRGVLRGVTFLLRREGDHEFLEVPEAQEDVEATFPVHQPGNYSCSYRTDGEGALSEPSATVTIEELAAPPPPVLMHHGESSQVLHPGNKVTLTCVAPLSGVDFQLRRGEKELLVPRSSTSPDRIFFHLNAVALGDGGHYTCRYRLHDNQNGWSGDSAPVELILSDETLPAPEFSPEPESGRALRLRCLAPLEGARFALVREDRGGRRVHRFQSPAGTEALFELHNISVADSANYSCVYVDLKPPFGGSAPSERLELHVDGPPPRPQLRATWSGAVLAGRDAVLRCEGPIPDVTFELLREGETKAVKTVRTPGAAANLELIFVGPQHAGNYRCRYRSWVPHTFESELSDPVELLVAES

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 alpha-1-B glycoprotein
别名 A1BG, A1B, Alpha-1-B glycoprotein, Alpha 1B glycoprotein, alpha 1 B glycoprotein
计算分子量 54 kDa
观测分子量 65 kDa
GenBank蛋白编号BC035719
基因名称 A1BG
Gene ID (NCBI) 1
RRIDAB_10640441
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP04217
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Alpha-1B-glycoprotein (A1BG) is a plasma protein and is a member of the immunoglobulin superfamily. A1BG contains 474 amino acids and has 5 intrachain disulfide bonds. It has been reported that cysteine-rich secretory protein 3 (CRISP-3) is a specific and high-affinity ligand of A1BG. A1BG-CRISP-3 complex displays a similar function in protecting the circulation from a potentially harmful effect of free CRISP3 (PMID: 15461460).

实验方案

Product Specific Protocols
IF protocol for Alpha 1B-Glycoprotein antibody 14181-1-APDownload protocol
IHC protocol for Alpha 1B-Glycoprotein antibody 14181-1-APDownload protocol
WB protocol for Alpha 1B-Glycoprotein antibody 14181-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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