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验证数据展示

  • WB analysis using 84814-1-RR

    WB analysis using 84814-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84814-1-RR (6*his,His tag antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IF Staining of HEK-293 using 84814-1-RR

    IF Staining of HEK-293 using 84814-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using 6*his,His tag antibody (84814-1-RR, Clone: 241261A7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • FC experiment of Transfected HEK-293T using 84814-1-RR

    FC experiment of Transfected HEK-293T using 84814-1-RR

    1x10^6 Transfected HEK-293T cells were intracellularly stained with 0.25 ug 6*his,His tag Recombinant antibody (84814-1-RR, Clone:241261A7) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), and 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 84814-1-RR

    Affinity and Kinetic Characterization of 84814-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84814-1-RR against a protein with N-terminal 6*His Tag were performed. The affinity constant is 1.69 nM.

  • Affinity and Kinetic Characterization of 84814-1-RR

    Affinity and Kinetic Characterization of 84814-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84814-1-RR against a protein with C-terminal 6*His Tag were performed. The affinity constant is 26.1 nM.

6*his,His tag Recombinant antibody

6*his,His tag Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 84814-1-RR

产品说明书

CloneNo. 241261A7

宿主/亚型

Rabbit / IgG

种属反应性

recombinant protein

应用

WB, IF/ICC, FC (Intra), ELISA

别名

6*His, His-Tag, 6*His, His Tag, His Tag, 6XHis, 6histag

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inTransfected HEK-293T cells
Positive IF/ICC detected inHEK-293 cells
Positive FC (Intra) detected inTransfected HEK-293T cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84814-1-RR targets 6*his,His tag in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with recombinant protein samples.

经测试应用 WB, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 recombinant protein
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 6*His, His Tag
别名 6*His, His-Tag, 6*His, His Tag, His Tag, 6XHis, 6histag
计算分子量 1 kDa
基因名称 His Tag
Gene ID (NCBI) 5
RRIDAB_3672138
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. His tag is often used for affinity purification and binding assays. Expressed His-tagged proteins can be purified and detected easily because the string of histidine residues binds to several types of immobilized metal ions, including nickel, cobalt and copper, under specific buffer conditions. The antibody reacts to the 6*His tagged protein.

实验方案

Product Specific Protocols
WB protocol for 6*his,His tag antibody 84814-1-RRDownload protocol
IF protocol for 6*his,His tag antibody 84814-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols