验证数据展示
经过测试的应用
| Positive WB detected in | Transfected HEK-293T cells |
| Positive IF/ICC detected in | HEK-293 cells |
| Positive FC (Intra) detected in | Transfected HEK-293T cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 5 publications below |
产品信息
84814-1-RR targets 6*His, His-Tag in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with recombinant protein samples.
| 经测试应用 | WB, IF/ICC, FC (Intra), ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | recombinant protein |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | 6*His, His-Tag |
| 别名 | 6*His, His Tag, His Tag, 241261A7, 6*His, 6*his,His tag |
| 计算分子量 | 1 kDa |
| 基因名称 | |
| Gene ID (NCBI) | |
| RRID | AB_3672138 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purfication |
| UNIPROT ID | HISTAG |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. His tag is often used for affinity purification and binding assays. Expressed His-tagged proteins can be purified and detected easily because the string of histidine residues binds to several types of immobilized metal ions, including nickel, cobalt and copper, under specific buffer conditions. The antibody reacts to the 6*His tagged protein.
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for 6*His, His-Tag antibody 84814-1-RR | Download protocol |
| IF protocol for 6*His, His-Tag antibody 84814-1-RR | Download protocol |
| WB protocol for 6*His, His-Tag antibody 84814-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Virol GP2a I118 and GP4 D43 play critical roles in the attachment of PRRSV to the CD163 receptor: implications for anti-PRRSV infection targets | ||
PLoS Pathog Two receptor-targeting mechanisms of lambda-like siphophage Gifsy-1 of Salmonella Typhimurium. | ||
Clin Transl Med DNA-mediated UCP1 overexpression in adipose tissue: A promising anti-obesity gene therapy. | ||
Vet Res PotD contributes to Streptococcus suis-induced blood-brain barrier disruption by regulating arcA transcription. | ||
Brain Behav Immun Targeting AARS1-dependent lactylation improves neuronal process plasticity and mitigates cognitive deficits in sepsis-associated encephalopathy. | ||
J Nanobiotechnology CD93-targeted resveratrol-loaded PLGA nanoparticles remodel CD8⁺ T cell metabolism through AIF-mediated oxidative phosphorylation to overcome lung cancer immunotherapy resistance. |
