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验证数据展示

  • FC experiment of Transfected HEK-293T using AY7-FcA98450

    FC experiment of Transfected HEK-293T using AY7-FcA98450

    1x10^6 transfected HEK-293T cells or untransfected HEK-293T cells were intracellularly stained with 0.25 ug APC-Cyanine7 Anti-6*His, His-Tag Rabbit RecAb (AY7-FcA98450, Clone: 251107A1). Cells were incubated with MonoZero™ Monocytes blocking Reagent (PF00020) prior to staining. Cells were fixed and permeabilized with Intracellular Flow Cytometry Fixation & Permeabilization Buffer Kit (PF00019).

  • FC experiment of Transfected HEK-293T using AY7-FcA98450

    FC experiment of Transfected HEK-293T using AY7-FcA98450

    1x10^6 transfected HEK-293T cells were intracellularly stained with 0.25 ug APC-Cyanine7 Anti-6*His, His-Tag Rabbit RecAb (AY7-FcA98450, Clone: 251107A1) (red) or 0.25 ug FcZero-rAb™ APC-Cyanine7 Rabbit IgG Isotype Control Recombinant Antibody (AY7-FcA98136, Clone: 240953C9) (blue). 1x10^6 untransfected HEK-293T cells were intracellularly stained with 0.25 ug APC-Cyanine7 Anti-6*His, His-Tag Rabbit RecAb (AY7-FcA98450, Clone:251107A1) (black,dashed). Cells were incubated with MonoZero™ Monocytes blocking Reagent (PF00020) prior to staining. Cells were fixed and permeabilized with Intracellular Flow Cytometry Fixation & Permeabilization Buffer Kit (PF00019).

  • Zero Fc receptor binding
  • Site-specific conjugation
  • One universal isotype

FcZero-rAb® APC-Cyanine7 Anti-6*His, His-Tag Rabbit Recombinant Antibody

6*His, His-Tag FcZero-rAb® Recombinant Antibody for FC (Intra)
Cat No. AY7-FcA98450

产品说明书

CloneNo. 251107A1

宿主/亚型

Rabbit / IgG

种属反应性

n/a

应用

FC (Intra)

别名

6*His, His Tag, His Tag, 6*His, 6*his tag, 6*his,His tag

缓冲液配方:  PBS, Azide, BSA
PBS, Azide, BSA
偶联物:  APC-Cyanine7
规格价格库存

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经过测试的应用

Positive FC (Intra) detected inTransfected HEK-293T cells

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

AY7-FcA98450 targets 6*His, His-Tag in FC (Intra) applications and shows reactivity with n/a samples.

经测试应用 FC (Intra) Application Description
经测试反应性 n/a
免疫原

unknow

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 6*His, His-Tag
别名 6*His, His Tag, His Tag, 6*His, 6*his tag, 6*his,His tag
计算分子量 1 kDa
基因名称
Gene ID (NCBI)
RRIDAB_3746714
偶联类型 APC-Cyanine7 Fluorescent Dye
最大激发/发射波长650 nm / 778 nm
激发激光Red Laser (633 nm)
形式Liquid
纯化方式Protein A purification
UNIPROT IDHISTAG
储存缓冲液 PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
储存条件Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. His-tag is often used for affinity purification and binding assays. Expressed His-tagged proteins can be purified and detected easily because the string of histidine residues binds to several types of immobilized metal ions, including nickel, cobalt and copper, under specific buffer conditions. The His-tag antibody is a useful tool for monitoring of the His-tagged proteins, and recognizes His-tags placed at N-terminal, C-terminal, and internal regions of fusion proteins expressed in bacteria, insect, and mammalian cells.

实验方案

Product Specific Protocols
FC protocol for APC-Cyanine7 6*His, His-Tag antibody AY7-FcA98450Download protocol
Standard Protocols
Click here to view our Standard Protocols
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