验证数据展示
经过测试的应用
| Positive WB detected in | mouse IMCD3 cells (RNAi), A431 cells, K-562 cells, NIH/3T3 cells |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human lung cancer tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse brain tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
12381-1-AP targets 14-3-3 GAMMA-Specific in WB, IHC, IF-P, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat, canine samples.
| 经测试应用 | WB, IHC, IF-P, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IP, CoIP |
| 经测试反应性 | human, mouse, rat, canine |
| 文献引用反应性 | human, rat |
| 免疫原 | 14-3-3 GAMMA-Specific fusion protein Ag3047 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, gamma polypeptide |
| 别名 | 14-3-3 GAMMA, YWHAG, 14 3 3 protein gamma, 14 3 3 protein gamma subtype, 14 3 3GAMMA |
| 计算分子量 | 247 aa, 28 kDa |
| 观测分子量 | 28-33 kDa |
| GenBank蛋白编号 | BC020963 |
| 基因名称 | 14-3-3 gamma |
| Gene ID (NCBI) | 7532 |
| RRID | AB_2217937 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P61981 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
14-3-3 gamma (also known as YWHAG) is a member of 14-3-3 proteins which were the first phosphoserine/phosphothreonine-binding proteins to be discovered. 14-3-3 family members interact with a wide spectrum of proteins and possess diverse functions. Mammals express seven distinct 14-3-3 isoforms (gamma, epsilon, beta, zeta, sigma, theta, tau) that form multiple homo- and hetero- dimmers. 14-3-3 proteins display the highest expression levels in the brain, and have been implicated in several neurodegenerative diseases, including Alzheimer's disease and amyotrophic lateral sclerosis. This antibody specifically recognizes gamma isoform of 14-3-3.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for 14-3-3 GAMMA-Specific antibody 12381-1-AP | Download protocol |
| IHC protocol for 14-3-3 GAMMA-Specific antibody 12381-1-AP | Download protocol |
| IF protocol for 14-3-3 GAMMA-Specific antibody 12381-1-AP | Download protocol |
| IP protocol for 14-3-3 GAMMA-Specific antibody 12381-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun Endonuclease G promotes autophagy by suppressing mTOR signaling and activating the DNA damage response. | ||
Biochim Biophys Acta Mol Cell Biol Lipids Human adipocyte differentiation and composition of disease-relevant lipids are regulated by miR-221-3p. | ||
Proteomics Proteome analysis of porcine epidemic diarrhea virus (PEDV)-infected vero cells. | ||
J Exp Clin Cancer Res 14-3-3ζ inhibits heme oxygenase-1 (HO-1) degradation and promotes hepatocellular carcinoma proliferation: involvement of STAT3 signaling. | ||
Autophagy YWHA/14-3-3 proteins recognize phosphorylated TFEB by a noncanonical mode for controlling TFEB cytoplasmic localization. | ||
J Biochem Mol Toxicol miR-200a-3p inhibits the PDGF-BB-induced proliferation of VSMCs by affecting their phenotype-associated proteins |
