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验证数据展示

  • WB analysis using 66946-1-Ig (same clone as 66946-1-PBS)

    WB analysis using 66946-1-Ig (same clone as 66946-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66946-1-Ig (14-3-3E antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66946-1-PBS in a different storage buffer formulation.

  • IHC staining of rat liver using 66946-1-Ig (same clone as 66946-1-PBS)

    IHC staining of rat liver using 66946-1-Ig (same clone as 66946-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 66946-1-Ig (14-3-3E antibody) at dilution of 1:1 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66946-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with YWHAE antibody included
  • IHC staining of rat liver using 66946-1-Ig (same clone as 66946-1-PBS)

    IHC staining of rat liver using 66946-1-Ig (same clone as 66946-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 66946-1-Ig (14-3-3E antibody) at dilution of 1:1 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66946-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with YWHAE antibody included

14-3-3E Monoclonal antibody, PBS Only

14-3-3E Monoclonal Antibody for WB, IHC, ELISA
Cat No. 66946-1-PBS

产品说明书

CloneNo. 3A3A4

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

14-3-3 epsilon, YWHAE, 14-3-3 protein epsilon, 14-3-3epsilon

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy YWHAE Ready-To-Use IHC Kit. YWHAE primary antibody included.

产品信息

66946-1-PBS targets 14-3-3E in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag25186

Product name: Recombinant human 14-3-3E protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-255 aa of BC000179

Sequence: MDDREDLVYQAKLAEQAERYDEMVESMKKVAGMDVELTVEERNLLSVAYKNVIGARRASWRIISSIEQKEENKGGEDKLKMIREYRQMVETELKLICCDILDVLDKHLIPAANTGESKVFYYKMKGDYHRYLAEFATGNDRKEAAENSLVAYKAASDIAMTELPPTHPIRLGLALNFSVFYYEILNSPDRACRLAKAAFDDAIAELDTLSEESYKDSTLIMQLLRDNLTLWTSDMQGDGEEQNKEALQDVEDENQ

种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon polypeptide
别名 14-3-3 epsilon, YWHAE, 14-3-3 protein epsilon, 14-3-3epsilon
计算分子量 255 aa, 29 kDa
观测分子量 28-32 kDa
GenBank蛋白编号BC000179
基因名称 14-3-3E
Gene ID (NCBI) 7531
RRIDAB_2882270
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP62258
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

14-3-3 epsilon (also known as YWHAE) is a member of 14-3-3 proteins which were the first phosphoserine/phosphothreonine-binding proteins to be discovered. 14-3-3 family members interact with a wide spectrum of proteins and possess diverse functions. Mammals express seven distinct 14-3-3 isoforms (gamma, epsilon, beta, zeta, sigma, theta, tau) that form multiple homo- and hetero- dimmers. 14-3-3 proteins display the highest expression levels in the brain, and have been implicated in several neurodegenerative diseases, including Alzheimer's disease and amyotrophic lateral sclerosis.

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