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验证数据展示

  • WB analysis using 66061-1-Ig (same clone as 66061-1-PBS)

    WB analysis using 66061-1-Ig (same clone as 66061-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66061-1-Ig (14-3-3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66061-1-PBS in a different storage buffer formulation.

  • IP experiment of rat brain using 66061-1-Ig (same clone as 66061-1-PBS)

    IP experiment of rat brain using 66061-1-Ig (same clone as 66061-1-PBS)

    IP result of anti-14-3-3 (IP:66061-1-Ig, 4ug; Detection:66061-1-Ig 1:20000) with rat brain tissue lysate 1080 ug. This data was developed using the same antibody clone with 66061-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 66061-1-Ig (same clone as 66061-1-PBS)

    IHC staining of human lung cancer using 66061-1-Ig (same clone as 66061-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66061-1-Ig (14-3-3 Antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66061-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 66061-1-Ig (same clone as 66061-1-PBS)

    IHC staining of human lung cancer using 66061-1-Ig (same clone as 66061-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66061-1-Ig (14-3-3 Antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66061-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 66061-1-Ig (same clone as 66061-1-PBS)

    IF Staining of HepG2 using 66061-1-Ig (same clone as 66061-1-PBS)

    Immunofluorescent analysis of HepG2 cells using 66061-1-Ig (14-3-3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG (H+L). This data was developed using the same antibody clone with 66061-1-PBS in a different storage buffer formulation.

14-3-3 Monoclonal antibody, PBS Only

14-3-3 Monoclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 66061-1-PBS

产品说明书

CloneNo. 2E10A3

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

YWHAQ, 14-3-3 protein T-cell, 14-3-3 theta, 14-3-3σ, 1C5

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

66061-1-PBS targets 14-3-3 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat, pig
免疫原

CatNo: Ag6055

Product name: Recombinant human 14-3-3 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-245 aa of BC056867

Sequence: MEKTELIQKAKLAEQAERYDDMATCMKAVTEQGAELSNEERNLLSVAYKNVVGGRRSAWRVISSIEQKTDTSDKKLQLIKDYREKVESELRSICTTVLELLDKYLIANATNPESKVFYLKMKGDYFRYLAEVACGDDRKQTIDNSQGAYQEAFDISKKEMQPTHPIRLGLALNFSVFYYEILNNPELACTLAKTAFDEAIAELDTLNEDSYKDSTLIMQLLRDNLTLWTSDSAGEECDAAEGAEN

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, theta polypeptide
别名 YWHAQ, 14-3-3 protein T-cell, 14-3-3 theta, 14-3-3σ, 1C5
计算分子量 28 kDa
观测分子量 31 kDa, 28 kDa
GenBank蛋白编号BC056867
基因名称 14-3-3 theta
Gene ID (NCBI) 10971
RRIDAB_11042772
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDP27348
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

14-3-3 proteins interact with a wide spectrum of proteins and possess diverse functions. Mammals express seven distinct 14-3-3 isoforms (gamma, epsilon, beta, zeta, sigma, theta, tau) that form multiple homo- and hetero- dimmers. 14-3-3 proteins display the highest expression levels in the brain, and have been implicated in several neurodegenerative diseases, including Alzheimer's disease and amyotrophic lateral sclerosis.

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