验证数据展示
经过测试的应用
| Positive FC detected in | human PBMCs |
Planning an IHC experiment? We recommend our IHCeasy CD20 Ready-To-Use IHC Kit. CD20 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Flow Cytometry (FC) | FC : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
SF65575-1-MR targets CD20 in FC applications and shows reactivity with human, cynomolgus monkey samples.
| 经测试应用 | FC Application Description |
| 经测试反应性 | human, cynomolgus monkey |
| 免疫原 |
Human tonsillar B cells 种属同源性预测 |
| 宿主/类型 | Mouse / scFv |
| 抗体类别 | Recombinant |
| 产品类型 | Single-chain variable fragment antibody (scFv) |
| 全称 | membrane-spanning 4-domains, subfamily A, member 1 |
| 别名 | 2H7, B lymphocyte antigen CD20, B1, B-lymphocyte antigen CD20, B-lymphocyte surface antigen B1 |
| 计算分子量 | 297 aa, 33 kDa |
| GenBank蛋白编号 | BC002807 |
| 基因名称 | CD20 |
| Gene ID (NCBI) | 931 |
| RRID | AB_3750140 |
| 偶联类型 | Unconjugated |
| 来源 | Anti-Human CD20 scFv from clone 2H7, consisting of VH-GGGGS linker-VL (N- to C-terminus), with a His-C tag at the C-terminus. |
| 形式 | Liquid |
| 纯化方式 | Affinity purification |
| 储存缓冲液 | 50mM HEPES, 150mM NaCl, 5mM EDTA, 0.1% CHAPS, 10% Sucrose and 0.1% Proclin300, pH 7.3. |
| 储存条件 | Store at 2-8°C. Stable for one year after shipment. |
背景介绍
CD20 is a 33-37 kDa transmembrane phosphoprotein belonging to the membrane-spanning 4A family (PMID: 3260267; 16785532). CD20 is a B-lymphocyte surface molecule that is widely expressed during B-cell ontogeny, from early pre-B-cell developmental stages until final differentiation into plasma cells (PMID: 7524522). CD20 functions as calcium-permeable cation channel (PMID: 7684739). It is involved in the regulation of B-cell activation, proliferation and differentiation (PMID: 7524522).
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for CD20 antibody SF65575-1-MR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
