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POLA2 Recombinant Matched Antibody Pair, PBS Only

Host / Isotype

Capture: Rabbit / IgG

Detection: Rabbit / IgG

Reactivity

Capture: human

Detection: human

Applications

Sandwich ELISA

Range

Sandwich ELISA: 1.56-50 ng/mL

Cat no : MP02735-2

Synonyms

DNA polymerase alpha subunit B

Validation Data Gallery

  • Sandwich ELISA standard curve of MP02735-2, Human POLA2 Recombinant Matched Antibody Pair - PBS only. 86856-5-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag0271. 86856-4-PBS was HRP conjugated as the detection antibody. Range: 1.56-50 ng/mL.

    Sandwich ELISA standard curve of MP02735-2

    Sandwich ELISA standard curve of MP02735-2, Human POLA2 Recombinant Matched Antibody Pair - PBS only. 86856-5-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag0271. 86856-4-PBS was HRP conjugated as the detection antibody. Range: 1.56-50 ng/mL.

  • The mean POLA2 concentration was determined to be 2,818.00 ng/mL in HeLa cell extract based on a 1.20 mg/mL extract load, 1,487.00 ng/mL in MCF-7 cell extract based on a 1.20 mg/mL extract load, 198.00 ng/mL in HepG2 cell extract based on a 1.20 mg/mL extract load and 873.00 ng/mL in A549 cell extract based on a 1.20 mg/mL extract load.

    Sandwich ELISA sample test of MP02735-2

    The mean POLA2 concentration was determined to be 2,818.00 ng/mL in HeLa cell extract based on a 1.20 mg/mL extract load, 1,487.00 ng/mL in MCF-7 cell extract based on a 1.20 mg/mL extract load, 198.00 ng/mL in HepG2 cell extract based on a 1.20 mg/mL extract load and 873.00 ng/mL in A549 cell extract based on a 1.20 mg/mL extract load.

Tested Applications

Sandwich ELISA

Recommended dilution

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

产品信息

MP02735-2 targets POLA2 in immunoassays as a matched antibody pair. Validated in Sandwich ELISA.

Range: 1.56-50 ng/mL (Sandwich ELISA);

Capture antibody: POLA2 Recombinant monoclonal antibody, PBS Only (Capture) 86856-5-PBS (251924C8). 100 μg. Concentration 1 mg/ml.

Detection antibody: POLA2 Recombinant monoclonal antibody, PBS Only (Detector) 86856-4-PBS (251924A9). 100 μg. Concentration 1 mg/ml.

Alternative POLA2 matched antibody pairs: MP02735-1

Matched pair protein standard: Ag0271

Unconjugated rabbit recombinant monoclonal antibody pair in PBS only storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

Matched antibody pairs are designed for use in a variety of assays and platforms that require matched antibody pairs. These include:
Sandwich ELISA
Multiplex bead-based arrays
FRET/TR-FRET/HTRF
Simoa® immunoassays (registered trademark of Quanterix, Inc)
AlphaLISA® (registered trademark of PerkinElmer, Inc)
Meso Scale Discovery® (registered trademark of Meso Scale Diagnostics, LLC)
ELLA™ Automated Immunoassay (registered trademark of Bio-Techne Corporation)

Please note we have not tested this product in the above platforms, they are listed for suggestion only as applications for matched antibody pairs. Antibody use should be optimized for each application and assay.

Catalog Number 86856-5-PBS
Tested Reactivity human
Immunogen POLA2 fusion protein Ag0271
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name polymerase (DNA directed), alpha 2 (70kD subunit)
Synonyms
GenBank accession numberBC001347
Gene symbol POLA2
UniprotQ14181
Gene ID (NCBI) 23649
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
Catalog Number 86856-4-PBS
Tested Reactivity human
Immunogen POLA2 fusion protein Ag0271
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name polymerase (DNA directed), alpha 2 (70kD subunit)
Synonyms
GenBank accession numberBC001347
Gene symbol POLA2
UniprotQ14181
Gene ID (NCBI) 23649
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
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