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IL-22RA2 Recombinant Matched Antibody Pair, PBS Only

Host / Isotype

Capture: Rabbit / IgG

Detection: Rabbit / IgG

Reactivity

Capture: human

Detection: human

Applications

Sandwich ELISA

Range

Sandwich ELISA: 0.781-50 ng/mL

Cat no : MP02591-1

Synonyms

IL22RA2, CRF2 10, CRF2 S1, CRF2-10, CRF2-S1

Validation Data Gallery

  • Sandwich ELISA standard curve of MP02591-1, Human IL-22RA2 Recombinant Matched Antibody Pair - PBS only. 86665-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg2825. 86665-1-PBS was HRP conjugated as the detection antibody. Range: 0.781-50 ng/mL.

    Sandwich ELISA standard curve of MP02591-1

    Sandwich ELISA standard curve of MP02591-1, Human IL-22RA2 Recombinant Matched Antibody Pair - PBS only. 86665-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg2825. 86665-1-PBS was HRP conjugated as the detection antibody. Range: 0.781-50 ng/mL.

  • The mean IL-22RA2 concentration was determined to be 9.02 ng/mL in U-937 cell extract based on a 2.60 mg/mL extract load and 51.68 ng/mL in Raji cell extract based on a 1.30 mg/mL extract load.

    Sandwich ELISA sample test of MP02591-1

    The mean IL-22RA2 concentration was determined to be 9.02 ng/mL in U-937 cell extract based on a 2.60 mg/mL extract load and 51.68 ng/mL in Raji cell extract based on a 1.30 mg/mL extract load.

  • Plasma of six individual healthy human donors was measured. The IL-22RA2 concentration of detected samples was determined to be 0.85 ng/mL with a range of 0.38-2.04 ng/mL.

    Sandwich ELISA sample test of MP02591-1

    Plasma of six individual healthy human donors was measured. The IL-22RA2 concentration of detected samples was determined to be 0.85 ng/mL with a range of 0.38-2.04 ng/mL.

Tested Applications

Sandwich ELISA

Recommended dilution

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

产品信息

MP02591-1 targets IL-22RA2 in immunoassays as a matched antibody pair. Validated in Sandwich ELISA.

Range: 0.781-50 ng/mL (Sandwich ELISA);

Capture antibody: IL22RA2 Recombinant monoclonal antibody, PBS Only (Capture) 86665-2-PBS (250314A8). 100 μg. Concentration 1 mg/ml.

Detection antibody: IL22RA2 Recombinant monoclonal antibody, PBS Only (Detector) 86665-1-PBS (250314G9). 100 μg. Concentration 1 mg/ml.

Matched pair protein standard: Eg2825

Unconjugated rabbit recombinant monoclonal antibody pair in PBS only storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

Matched antibody pairs are designed for use in a variety of assays and platforms that require matched antibody pairs. These include:
Sandwich ELISA
Multiplex bead-based arrays
FRET/TR-FRET/HTRF
Simoa® immunoassays (registered trademark of Quanterix, Inc)
AlphaLISA® (registered trademark of PerkinElmer, Inc)
Meso Scale Discovery® (registered trademark of Meso Scale Diagnostics, LLC)
ELLA™ Automated Immunoassay (registered trademark of Bio-Techne Corporation)

Please note we have not tested this product in the above platforms, they are listed for suggestion only as applications for matched antibody pairs. Antibody use should be optimized for each application and assay.

Catalog Number 86665-2-PBS
Tested Reactivity human
Immunogen IL22RA2 fusion protein Eg2825
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name interleukin 22 receptor, alpha 2
Synonyms IL-22RA2, CRF2 10, CRF2 S1, CRF2-10, CRF2-S1
GenBank accession numberNM_181309.1
Gene symbol IL-22RA2
UniprotQ969J5-2
Gene ID (NCBI) 116379
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
Catalog Number 86665-1-PBS
Tested Reactivity human
Immunogen IL22RA2 fusion protein Eg2825
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name interleukin 22 receptor, alpha 2
Synonyms IL-22RA2, CRF2 10, CRF2 S1, CRF2-10, CRF2-S1
GenBank accession numberNM_181309.1
Gene symbol IL-22RA2
UniprotQ969J5-2
Gene ID (NCBI) 116379
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
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