Validation Data Gallery
Technical Specifications
| GeneID | 5155 |
| Species | Human |
| Expression | HEK293 |
| Activity | 0.3-3 ng/mL |
| Specific Activity | minimally 5.00 x 106 IU/mg |
| Purity | >95% |
| Endotoxin | <1 EU/μg |
| Accession Number | P31240 |
| Molecular Mass | 14 and 36 kDa reduced, 29 to 32 kDa non-reduced, homodimer, glycosylated |
| Formulation | 50mM Acetate pH 3.5 + 500mM NaCl, See Certificate of Analysis for details |
| Species Reactivity | human,mouse |
Stability and Reconstitution
| Stability and Storage | Product Form | Temperature Conditions | Storage Time (From Date of Receipt) |
|---|---|---|---|
| Lyophilized | -20°C to -80°C | Until Expiry Date | |
| Lyophilized | Room Temperature | 2 weeks | |
| Reconstituted as per CofA | -20°C to -80°C | 6 months | |
| Reconstituted as per CofA | 4°C | 1 week | |
| Avoid repeated freeze-thaw cycles. | |||
| Reconstitution | Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to 0.2 mg/mL in sterile 1x PBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA). Gently swirl or tap vial to mix. |
Background
PDGFbb is a member of the platelet-derived growth family factor. Four members of this family are mitogenic factors for cells of mesenchymal origin, and they are characterized by a motif of eight cysteines. PDGFbb is required for normal proliferation and recruitment of pericytes and vascular smooth muscle cells in the central nervous system, skin, and placenta among others. PDGFbb plays a role in blood vessel development. It also implements its role in wound healing (PMID: 29266203; 28851707; 28556368).
Synonyms
Becaplermin, c sis, FLJ12858, PDGF 2, PDGF subunit B, PDGF2, PDGFB, PDGFbb, Proto oncogene c Sis, SIS, SSV
Publications
| Species | Title |
|---|---|
Int J Biol Sci Colchicine Blocks Abdominal Aortic Aneurysm Development by Maintaining Vascular Smooth Muscle Cell Homeostasis | |
J Neuroinflammation SU16f inhibits fibrotic scar formation and facilitates axon regeneration and locomotor function recovery after spinal cord injury by blocking the PDGFRβ pathway. | |
Phytomedicine Bergenin alleviates proliferative arterial diseases by modulating glucose metabolism in vascular smooth muscle cells | |
Br J Pharmacol A novel HSF1 activator ameliorates nonalcoholic steatohepatitis by stimulating mitochondrial adaptive oxidation | |
J Ethnopharmacol A modified Fangji Huangqi decoction ameliorates pulmonary artery hypertension via phosphatidylinositide 3-kinases/protein kinase B-mediated regulation of proliferation and apoptosis of smooth muscle cells in vitro and in vivo | |
Cells Pericyte-to-Endothelial Cell Communication via Tunneling Nanotubes Is Disrupted by a Diol of Docosahexaenoic Acid |
