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Validation Data Gallery

  • Purity of recombinant human IL-12 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

    Purity of recombinant human IL-12 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

  • Recombinant human IL-12 (HZ-1256) stimulates dose-dependent induction of alkaline phosphatase production in a HEK293 reporter cell line. Alkaline phosphatase production was assessed using pNPP as a chromogenic substrate. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 values range from 1-5 ng/mL.

    Recombinant human IL-12 (HZ-1256) stimulates dose-dependent induction of alkaline phosphatase production in a HEK293 reporter cell line. Alkaline phosphatase production was assessed using pNPP as a chromogenic substrate. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 values range from 1-5 ng/mL.

  • Proteintech IL-12 (HZ-1256) demonstrates equivalent EC50 and alkaline phosphatase induction to leading competitors. Recombinant human IL-12 stimulates dose-dependent induction of alkaline phosphatase production in a HEK293 reporter cell line. Alkaline phosphatase production was assessed using pNPP as a chromogenic substrate. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 values range from 1-5 ng/mL.

    Proteintech IL-12 (HZ-1256) demonstrates equivalent EC50 and alkaline phosphatase induction to leading competitors. Recombinant human IL-12 stimulates dose-dependent induction of alkaline phosphatase production in a HEK293 reporter cell line. Alkaline phosphatase production was assessed using pNPP as a chromogenic substrate. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 values range from 1-5 ng/mL.

  • HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human IL-12 protein

Cat No. HZ-1256
引用文献(12)

Activity

0.8-4 ng/mL

Specific Activity

minimally 3.00 x 106 IU/mg

Species Reactivity

human

Purity

>95%

Synonyms

CLMF, CLMF p35, IL 12 subunit p35, IL 12A, IL12, IL-12, IL12A, Interleukin 12 subunit alpha, NFSK, NKSF1, P35

Product type:  Research use only
规格价格库存

GMP-Grade HumanKine® Info

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Technical Specifications

GeneID 3592|3593
Species Human
Expression HEK293
Activity 0.8-4 ng/mL
Specific Activityminimally 3.00 x 106 IU/mg
Purity >95%
Endotoxin<1 EU/μg
Accession NumberP29459 (p35 subunit) P29460 (p40 subunit)
Molecular Mass 34 and 42 kDa reduced, 57 kDa non-reduced, heterodimer, glycosylated
Formulation 1x PBS, See Certificate of Analysis for details
Species Reactivity human

Stability and Reconstitution

Stability and StorageProduct FormTemperature ConditionsStorage Time
(From Date of Receipt)
Lyophilized-20°C to -80°CUntil Expiry Date
LyophilizedRoom Temperature2 weeks
Reconstituted as per CofA-20°C to -80°C6 months
Reconstituted as per CofA4°C1 week
Avoid repeated freeze-thaw cycles.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to 0.2 mg/mL in sterile 1x PBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA). Gently swirl or tap vial to mix.

Background

IL-12 is a heterodimeric cytokine composed of two glycosylated and disulfide-linked subunits (p40 cysteine-linked to p35). IL-12 is a potent regulator of cell mediated immune responses and it induces IFN-gamma production by NK and T cells. It is produced by activated monocytes/macrophage cells, B lymphocytes, and connective tissue type mast cells (PMID: 24821971; 7612223).

Synonyms

CLMF, CLMF p35, IL 12 subunit p35, IL 12A, IL12, IL-12, IL12A, Interleukin 12 subunit alpha, NFSK, NKSF1, P35

Publications

SpeciesTitle

Nat Commun

Extracellular mRNA transported to the nucleus exerts translation-independent function.

Authors - Takeshi Tomita
View Article
mouse

Nat Commun

Early life gut microbiota sustains liver-resident natural killer cells maturation via the butyrate-IL-18 axis

Authors - Panpan Tian
View Article
human

Adv Sci (Weinh)

Electret-Inspired Charge-Injected Hydrogel for Scar-Free Healing of Bacterially Infected Burns Through Bioelectrical Stimulation and Immune Modulation

Authors - Mujie Liu
View Article
mouse

Cell Rep

In vivo G-CSF treatment activates the GR-SOCS1 axis to suppress IFN-γ secretion by natural killer cells

Authors - Xiangyu Zhao
View Article

Br J Pharmacol

Tyrosine kinase 2 inhibition by GL-V9 treats psoriasiform dermatitis via macrophage-helper T-cell modulation.

Authors - Leyi Chen
View Article
human

Front Immunol

SLAMF7 (CD319) enhances cytotoxic T-cell differentiation and sensitizes CD8(+) T cells to immune checkpoint blockade.

Authors - Jan-Erik Sander
View Article
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