Recombinant Human Fibronectin protein (rFc Tag)
种属
Human
纯度
>90 %, SDS-PAGE
标签
rFc Tag
生物活性
未测试
验证数据展示
产品信息
| 纯度 | >90 %, SDS-PAGE |
| 内毒素 | <0.1 EU/μg protein, LAL method |
| 生物活性 |
Not tested |
| 来源 | HEK293-derived Human Fibronectin protein Ser607-Pro1265 (Accession# P02751-15) with a rabbit IgG Fc tag at the C-terminus. |
| 基因ID | 2335 |
| 蛋白编号 | P02751-15 |
| 预测分子量 | 97.8 kDa |
| SDS-PAGE | 90-120 kDa, reducing (R) conditions |
| 组分 | Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization. |
| 复溶 | Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water. |
| 储存条件 |
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
|
| 运输条件 | The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature. |
背景信息
Fibronectin (FN1) is a high molecular weight glycoprotein which exists in both a soluble form in plasma (plasma FN1) and other body fluids and an insoluble form in the extracellular matrix (cellular FN1). Plasma FN1 (dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix. FN1 binds to cell surfaces through integrins and to various compounds including collagen, fibrin and heparin. It is involved in cell adhesion and migration processes including embryogenesis, wound healing, hemostasis, host defense, and metastasis.
参考文献:
1.Mosher, D F, and L T Furcht. The Journal of investigative dermatology vol. 77,2 (1981): 175-80. 2.Pankov, Roumen, and Kenneth M Yamada. Journal of cell science vol. 115,Pt 20 (2002): 3861-3. 3.Muro, Andres F et al. The Journal of cell biology vol. 162,1 (2003): 149-60.
