Recombinant Human P21 protein (rFc Tag)
种属
Human
纯度
>90 %, SDS-PAGE
标签
C-rFc
生物活性
未测试
验证数据展示
产品信息
| 纯度 | >90 %, SDS-PAGE |
| 内毒素 | <0.1 EU/μg protein, LAL method |
| 生物活性 |
Not tested |
| 来源 | HEK293-derived Human P21 protein Met1-Pro164 (Accession# P38936) with a rabbit IgG Fc tag at the C-terminus |
| 基因ID | 1026 |
| 蛋白编号 | P38936 |
| 预测分子量 | 44.4 kDa |
| SDS-PAGE | 17 kDa & 30-32 kDa & 45-50 kDa, reducing (R) condition |
| 组分 | Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization. |
| 复溶 | Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water. |
| 储存条件 |
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
|
| 运输条件 | The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature. |
背景信息
P21 (also known as CIP1, WAF1) is a cyclin-dependent kinase inhibitor. P21 binds to and inhibits the activity of cyclin-CDK2 or -CDK4 complexes, and thus functions as a regulator of cell cycle progression at the G1 phase. The expression of P21 is induced by wild-type but not mutant p53 protein, through which P21 mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. P21 can interact with proliferating cell nuclear antigen (PCNA), and plays a regulatory role in S phase DNA replication and DNA damage repair. P21 was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation.
参考文献:
1. Deiry WS. et al.(1994). Cancer Res. 54: 1169-74. 2. Daniel Lew. et al. (2008). Mol Biol Cell. 19: 65-77. 3. Abbas T. et al. (2009). Nat. Rev. Cancer. 9: 400-414. 4. Chang BD. et al. (2002). Proc. Natl. Acad. Sci. U. S. A. 99: 389-394. 5. provided by RefSeq, Jul 2008.
