Recombinant Mouse PSAP/Prosaposin protein (rFc Tag)

种属

Mouse

纯度

>90 %, SDS-PAGE

标签

rFc Tag

生物活性

未测试

Cat no : Eg3155

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Synonyms

Prosaposin, Psap, Saposin-A, Saposin-B, Saposin-B-Val



产品信息

纯度 >90 %, SDS-PAGE
内毒素 <0.1 EU/μg protein, LAL method
生物活性
Not tested
来源 HEK293-derived Mouse PSAP protein Ser17-Asn557 (Accession# Q61207) with a rabbit IgG Fc tag at the C-terminus.
基因ID 19156
蛋白编号 Q61207
预测分子量 85.8 kDa
SDS-PAGE 90-100 kDa, reducing (R) conditions
组分 Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
复溶 Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
储存条件
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
运输条件 The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

背景信息

Psap (Prosaposin) is a highly conserved glycoprotein that is involved in the metabolism of various sphingolipids within the cell and can be secreted into plasma, breast milk, or cerebrospinal fluid.Prosaposin proteins are proteolytically cleaved to produce four different polypeptides called saposin A, B, C, or D, which are required for the hydrolysis of certain sphingolipids by lysosomal hydrolases. required for the hydrolysis of certain sphingolipids by lysosomal hydrolases, and whose mutation results in lysosomal hydrolase deficiency and subsequent lysosomal storage disease. (PMID: 33197249) PSAP overexpression is associated with poor prognosis and promotes tumorigenesis and cancer stem cell proliferation in subcutaneous and in situ models of glioma. In patients with mesenchymal glioblastoma, PSAP is a marker of poor prognosis, as its high expression has been found to shorten patients' overall survival.

参考文献:

1.Lin ZH, et al. Brain. 2021;144(1):e3. 2. Wen Z, et al. Pathol Res Pract. 2022;238:154027. 3. Pankaj Sharma et al. Science. 2024; 383,190-200.

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