Recombinant Rat TNF-alpha protein (Myc Tag, His Tag)
种属
Rat
纯度
>80 %, SDS-PAGE
标签
Myc Tag, His Tag
生物活性
未测试
验证数据展示
产品信息
| 纯度 | >80 %, SDS-PAGE |
| 内毒素 | <0.1 EU/μg protein, LAL method |
| 生物活性 |
Not tested |
| 来源 | HEK293-derived Rat TNF-alpha protein Leu80-Leu235 (Accession# P16599) with a Myc tag and a His tag at the C-terminus. |
| 基因ID | 24835 |
| 蛋白编号 | P16599 |
| 预测分子量 | 22.4 kDa |
| SDS-PAGE | 21-23 kDa |
| 组分 | Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization. |
| 复溶 | Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water. |
| 储存条件 |
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
|
| 运输条件 | The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature. |
背景信息
TNF, as also known as TNF-alpha, or cachectin, is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. It is expressed as a 26 kDa membrane bound protein and is then cleaved by TNF-alpha converting enzyme (TACE) to release the soluble 17 kDa monomer, which forms homotrimers in circulation. It is produced chiefly by activated macrophages, although it can be produced by many other cell types such as CD4+ lymphocytes, NK cells, neutrophils, mast cells, eosinophils, and neurons. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. rat and human TNF-alpha share 79% amino acid sequence identity. Unlike human TNF-alpha, the rat form is glycosylated. In rat deficiency of this gene is associated with defects in response to bacterial infection, with defects in forming organized follicular dendritic cell networks and germinal centers, and with a lack of primary B cell follicles.
参考文献:
1. Agbanoma G. et al. (2012) J Immunol. 188: 1307-17. 2. Kriegler M. et al. (1988) Cell. 53: 45-53. 3. Theiss AL. et al. (2005) J Biol Chem. 280: 36099-109. 4. Swardfager W. et al. (2010) Biol Psychiatry. 68:930-41. 5. Locksley RM.et al. (2001) Cell. 104(4):487-501.
