Recombinant Human APP protein (rFc Tag)
种属
Human
纯度
>80 %, SDS-PAGE
标签
rFc Tag
生物活性
未测试
验证数据展示
产品信息
| 纯度 | >80 %, SDS-PAGE |
| 内毒素 | <0.1 EU/μg protein, LAL method |
| 生物活性 |
Not tested |
| 来源 | HEK293-derived Human APP protein Leu18-Lys687 (Accession# P05067-1) with a rabbit IgG Fc tag at the C-terminus. |
| 基因ID | 351 |
| 蛋白编号 | P05067-1 |
| 预测分子量 | 102.0 kDa |
| SDS-PAGE | 100-140 kDa, reducing (R) conditions |
| 组分 | Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization. |
| 复溶 | Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water. |
| 储存条件 |
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
|
| 运输条件 | The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature. |
背景信息
Aβ derives from APP via proteolytic cleavage by proteases called α-, β- and γ-secretase. The α-secretase cleavage precludes the formation of Aβ, while the β- and γ-cleavages generate APP components with amyloidogenic features. Amyloid beta A4 precursor protein(APP), encoded by APP gene which locate on human chromosome 21q, is a cell surface receptor and performs physiological functions on the surface of neurons relevant to neurite growth, neuronal adhesion and axonogenesis. APP expressed in all fetal tissues and is pronounced in brain, kidney, heart and spleen, but weak in liver. Defects in APP are the cause of Alzheimer disease type 1 (AD1).
参考文献:
1.Nguyen, Khue Vu. Biomolecular concepts vol. 6,1 (2015): 11-32. 2.Baumkötter, Frederik et al. The Journal of neuroscience : the official journal of the Society for Neuroscience vol. 34,33 (2014): 11159-72. 3.Mullan, M et al. Nature genetics vol. 1,5 (1992): 345-7.
