Recombinant Mouse LCAT protein (rFc Tag)

种属

Mouse

纯度

>90 %, SDS-PAGE

标签

rFc Tag

生物活性

未测试

Cat no : Eg2756

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Synonyms

1-alkyl-2-acetylglycerophosphocholine esterase, EC:2.3.1.43, EC:3.1.1.47, Lcat, Lecithin-cholesterol acyltransferase



产品信息

纯度 >90 %, SDS-PAGE
内毒素 <0.1 EU/μg protein, LAL method
生物活性
Not tested
来源 HEK293-derived Mouse LCAT protein Phe25-Glu438 (Accession# P16301) with a rabbit IgG Fc tag at the C-terminus.
基因ID 16816
蛋白编号 P16301
预测分子量 73.3 kDa
SDS-PAGE 80-110 kDa, reducing (R) conditions
组分 Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
复溶 Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
储存条件
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
运输条件 The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

背景信息

Lecithin-cholesterolacyltransferase (LCAT) is an enzyme that acts as a catalyst in plasma, is synthesized by the liver and released into the bloodstream, and exists either free or bound to lipoproteins. The main function of LCAT is to transfer unsaturated fatty acids at the C2 position of HDL lecithin to free cholesterol to generate hemolytic lecithin and cholesteryl esters. The main function of LCAT is to transfer unsaturated fatty acids from the C2 site of HDL lecithin to free cholesterol, resulting in the production of hemolysed lecithin and cholesteryl esters, which are catalyzed by LCAT in almost 70-80% of plasma cholesterol.Reduced or complete lack of normal function of LCAT leads to two autosomal recessive disorders, familial LCAT deficiency and fish-eye disease, respectively. (PMID: 35121343)

参考文献:

1. Yang K, et al. Biomed Pharmacother. 2022;147:112677. 2. Lin X, et al. J Adv Res. 2024;63:187-194. 3. Leal-Gonzalez R, et al. Obstet Med. 2021;14(3):193-196. 4. Mehta R, et al. Lipids Health Dis. 2021;20(1):70.

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