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Recombinant Mouse SEMA5A protein (rFc Tag) (HPLC verified)

种属

Mouse

纯度

>90 %, SDS-PAGE
>90 %, SEC-HPLC

标签

rFc Tag

生物活性

未测试

Cat no : Eg2689

Print datasheet

Synonyms

Semaf, Semaphorin 5A, Semaphorin-5A, SemF

验证数据展示

  • Purity of Recombinant Mouse SEMA5A was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Mouse SEMA5A was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

  • The purity of Mouse SEMA5A was greater than 90% as determined by SEC-HPLC.

    The purity of Mouse SEMA5A was greater than 90% as determined by SEC-HPLC.

产品信息

纯度 >90 %, SDS-PAGE
>90 %, SEC-HPLC
内毒素 <0.1 EU/μg protein, LAL method
生物活性 
Not tested
来源 HEK293-derived Mouse SEMA5A protein Glu23-Thr765 (Accession# Q3UPZ0) with a rabbit IgG Fc tag at the C-terminus.
基因ID 20356
蛋白编号 Q3UPZ0
预测分子量 109.6 kDa
SDS-PAGE 110-130 kDa, reducing (R) conditions
组分 Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
复溶 Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
储存条件
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
运输条件 The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

背景信息

Semaphorin 5A, is a member of the semaphorin family of proteins, which are known for their roles in axon guidance and the development of the nervous system.Sema5A is a bifunctional guidance cue that can exert both permissive and inhibitory properties toward developing axons and migrating cells. The protein is also implicated in the promotion of angiogenesis by increasing endothelial cell proliferation, migration, and inhibiting apoptosis.

参考文献:

1. Nagy GN. et al. (2024). Nat Commun.15(1):2723. 
2. Sadanandam A. et al. (2010). Microvasc Res. 79(1):1-9.
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