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Recombinant Mouse ACBP/DBI protein (rFc Tag) (HPLC verified)

种属

Mouse

纯度

>90 %, SDS-PAGE
>90%, SEC-HPLC

标签

rFc Tag

生物活性

未测试

Cat no : Eg2462

Print datasheet

Synonyms

ACBP, Acyl-CoA-binding protein, DBI, Diazepam-binding inhibitor, Endozepine

验证数据展示

  • Purity of Recombinant Mouse ACBP was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Mouse ACBP was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

  • The purity of Mouse ACBP/DBI was greater than 90% as determined by SEC-HPLC.

    The purity of Mouse ACBP/DBI was greater than 90% as determined by SEC-HPLC.

产品信息

纯度 >90 %, SDS-PAGE
>90%, SEC-HPLC
内毒素 <0.1 EU/μg protein, LAL method
生物活性 
Not tested
来源 HEK293-derived Mouse ACBP protein Met1-Ile87 (Accession# P31786) with a rabbit IgG Fc tag at the N-terminus.
基因ID 13167
蛋白编号 P31786
预测分子量 37.2 kDa
SDS-PAGE 35-40 kDa, reducing (R) conditions
组分 Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
复溶 Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
储存条件
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
运输条件 The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

背景信息

Acyl coenzyme A binding protein (ACBP), also known as diazepam-binding inhibitor (DBI), is a phylogenetically ancient protein present in some eubacteria and the entire eukaryotic radiation. In several eukaryotic phyla, ACBP/DBI transcends its intracellular function in fatty acid metabolism because it can be released into the extracellular space.Indeed, autophagy is tied to the atypical secretion of this leaderless protein that is predominantly present in the cytosol of nucleated cells. Once released into the extracellular space, ACBP/DBI then acts on gamma-aminobutyric acid (GABA) receptors to inhibit autophagy via autocrine, paracrine, and neuroendocrine pathways.

参考文献:

1.Montégut, Léa et al. (2023) Aging Cell.22(9):e13910. 
2.Motiño, Omar et al. (2022) Proc Natl Acad Sci U S A. 119(41):e2207344119. 
3.Alquier, Thierry et al. (2021) Trends Endocrinol Metab. 32(11):890-903.