Recombinant Human CD43 protein (rFc Tag)
种属
Human
纯度
>90 %, SDS-PAGE
标签
rFc Tag
生物活性
未测试
验证数据展示
产品信息
| 纯度 | >90 %, SDS-PAGE |
| 内毒素 | <0.1 EU/μg protein, LAL method |
| 生物活性 | Not tested |
| 来源 | HEK293-derived Human CD43 protein Ser20-Arg253 (Accession# P16150) with a rabbit IgG Fc tag at the C-terminus. |
| 基因ID | 6693 |
| 蛋白编号 | P16150 |
| 预测分子量 | 49.7 kDa |
| SDS-PAGE | 52-100 kDa, reducing (R) conditions |
| 组分 | Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization. |
| 复溶 | Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water. |
| 储存条件 |
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
|
| 运输条件 | The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature. |
背景信息
CD43, also named leukosialin, sialophorin, galactoglycoprotein, leukocyte sialoglycoprotein, SPN, and is a mucin-like type I transmembrane protein. In humans, it is expressed in haematopoietic cells, including T lymphocytes, monocytes, granulocytes, natural killer cells, platelets, and haematopoietic stem cells, with the exception of mature erythrocytes and B cell subpopulations. CD43 can both induce and protect against apoptosis, and can either promote or block cell adhesion. The unglycosylated form of CD43 migrates on SDS-PAGE with a molecular weight of 54 kDa (PMID: 2943740), whereas CD43 from different haematopoietic cell lines displays increased molecular weights since it is glycosylated with O-linked chains that differ in core structure and sialylation.
参考文献:
1. M Santamaría, et al. (1996) Cancer Res. 56(15):3526-9. 2. T J Brown, et al. (1996) J Biol Chem. 271(44):27686-95. 3. S R Carlsson, et al. (1986) J Biol Chem. 261(27):12779-86.