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Recombinant Mouse Erythropoietin/EPO protein (His Tag)

种属

Mouse

纯度

>90 %, SDS-PAGE

标签

His Tag

生物活性

未测试

Cat no : Eg0265

Print datasheet

Synonyms

EPO, erythropoietin

验证数据展示

  • Purity of Recombinant Mouse Erythropoietin was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Mouse Erythropoietin was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

产品信息

纯度 >90 %, SDS-PAGE
内毒素 <0.1 EU/μg protein, LAL method
生物活性 Not tested
来源 HEK293-derived Mouse Erythropoietin protein Ala27-Arg192 (Accession# P07321) with a His tag at the C-terminus.
基因ID 13856
蛋白编号 P07321
预测分子量 22.7 kDa
SDS-PAGE 32-45 kDa, reducing (R) conditions
组分 Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
复溶 Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
储存条件
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
运输条件 The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

背景信息

Erythropoietin (EPO) is a glycoprotein hormone that regulates the production of red blood cells and biosynthesis of hemoglobin. The predominant expression of this gene shifts from the liver during fetal development to kidney in adults, and and the secreted protein will travel through the blood stream to reach to the bone marrow to stimulate hematopoietic stem cell differentiation to RBC. EPO binds to the cognate EPO receptor (EPOR) on erythroid progenitor cells, thus preventing apoptosis and stimulating their differentiation and maturation into erythrocyte. However, EPO protein and its receptors have also been shown to be cytoprotective in extra-hematopoietic tissues including the retina tissue. A complete lack of erythropoietin causes embryonic lethal anemia in mice. The conditional inactivation of erythropoietin in adult mice results in a chronic, normocytic and normochromic anemia. 

参考文献:

1. Chateauvieux S. et al.(2011) Biochem Pharmacol. 15;82(10):1291-303.
2. Caprara C. et al.(2014) Mol Vis. 20:307-24.
3. Brines M. et al. (2004) Proc Natl Acad Sci U S A. 101(41):14907-12.
4. Dis Model Mech. et al. (2010) Dis Model Mech. 3(11-12):763-72.