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Recombinant Human B7-H4 protein (Myc Tag, His Tag)

种属

Human

纯度

>90 %, SDS-PAGE

标签

Myc Tag, His Tag

生物活性

未测试

Cat no : Eg0098

Print datasheet

Synonyms

VTCN1, B7 H4, B7 homolog 4, B7h.5, B7H4

验证数据展示

  • Purity of Recombinant Human B7-H4 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Human B7-H4 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

产品信息

纯度 >90 %, SDS-PAGE
内毒素 <0.1 EU/μg protein, LAL method
生物活性 
Not tested
来源 HEK293-derived Human B7-H4 protein Phe29-Ala258 (Accession# Q7Z7D3-1) with a Myc tag, His tag at the C-terminus.
基因ID 79679
蛋白编号 Q7Z7D3-1
预测分子量 30.3 kDa
SDS-PAGE 40-70 kDa, reducing (R) conditions
组分 Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
复溶 Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
储存条件
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
运输条件 The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

背景信息

B7‑H4, also named VTCN1, B7X, or B7S1, is a 282 amino acid protein, which contains 2 immunoglobulin-like domains and belongs to the immunoglobulin superfamily. B7‑H4 negatively regulates T-cell mediated immune response by inhibiting T-cell activation, proliferation, cytokine production and development of cytotoxicity. B7‑H4 is a single-pass type I membrane protein, which is over-expressed in breast, ovarian, endometrial, renal cell and non-small-cell lung cancers. B7-H4 is up-regulated on the surface of cancer cells and immunosuppressive tumor-associated macrophages (TAMs) in a variety of human cancers.

参考文献:

1. Wang JY, et al. (2020). Cell Immunol. 347:104008.
2. Yu J, et al. (2024). Cell. 187 (17): 4713-4732.e19. 
3. Smith JB, et al. (2014). Gynecol Oncol. 34(1):181-189. 
4. Tringler B, et al. (2006). Gynecol Oncol.100(1):44-52.
5. Daha N A, et al. (2012). Ann Rheum Dis. 71(4): 567–571.