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Conjugate
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验证数据展示

  • FC experiment of human peripheral blood platelets using CL405-65173

    FC experiment of human peripheral blood platelets using CL405-65173

    100 ul human platelet-rich plasma (diluted 1:2 with PBS) was surface stained with 5 ul CoraLite® Plus 405 Anti-Human CD41a (CL405-65173, Clone:HIP8) or CoraLite® Plus 405 Mouse IgG1 Isotype Control (CL405-65124). Cells were not fixed.

  • FC experiment of human peripheral blood platelets using CL405-65173

    FC experiment of human peripheral blood platelets using CL405-65173

    100 ul human platelet-rich plasma (diluted 1:2 with PBS) was surface stained with 5 ul CoraLite® Plus 405 Anti-Human CD41a (CL405-65173, Clone:HIP8) or CoraLite® Plus 405 Mouse IgG1 Isotype Control (CL405-65124). Cells were not fixed.

CoraLite® Plus 405 Anti-Human CD41 (HIP8)

CD41 Monoclonal Antibody for FC
Cat No. CL405-65173

产品说明书

CloneNo. HIP8

宿主/亚型

Mouse / IgG1, kappa

种属反应性

human, non-human primates

应用

FC

别名

CD41a, CD41B, GP2B, GPalpha IIb, GPIIb

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 405
规格价格库存

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经过测试的应用

Positive FC detected inhuman peripheral blood platelets
For other applications, we recommend the unconjugated version of this antibody, 65173-1-Ig

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC)FC : 5 ul per 10^6 cells in 100 μl suspension
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

产品信息

CL405-65173 targets CD41 in FC applications and shows reactivity with human, non-human primates samples.

经测试应用 FC Application Description
经测试反应性 human, non-human primates
免疫原 N/A 种属同源性预测
宿主/亚型 Mouse / IgG1, kappa
抗体类别 Monoclonal
产品类型 Antibody
全称 integrin, alpha 2b (platelet glycoprotein IIb of IIb/IIIa complex, antigen CD41)
别名 CD41a, CD41B, GP2B, GPalpha IIb, GPIIb
GenBank蛋白编号BC117443
基因名称 CD41a
Gene ID (NCBI) 3674
偶联类型 CoraLite® Plus 405 Fluorescent Dye
最大激发/发射波长399 nm / 422 nm
形式 Liquid
纯化方式Unknow
UNIPROT IDP08514
储存缓冲液 PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
储存条件Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

The integrins are a superfamily of cell adhesion receptors that bind to extracellular matrix ligands, cell-surface ligands, and soluble ligands (PMID: 17543136). They are transmembrane αβ heterodimers and at least 24 distinct integrin heterodimers are formed by the combination of 18 α and eight β known subunits (PMID: 17543136; 20029421). In addition to mediating cell adhesion, integrins also play important roles in modulating signal transduction pathways that control cellular responses including migration, proliferation, differentiation, and apoptosis (PMID: 19118207). Integrin alpha-IIb (ITGA2B, CD41) is expressed on platelets, megakaryocytes and some hematopoietic progenitor cells (PMID: 11934866). This protein undergoes post-translational cleavage to yield disulfide linked light beta (25 kDa) and heavy alpha (125 kDa) chains, which join with integrin beta3 (CD61) to form a receptor for fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin and vitronectin. CD41/CD61 is crucial for platelet aggregation through binding of soluble fibrinogen. The HIP8 clone antibody specifically binds to the alpha chain of CD41.

实验方案

Product Specific Protocols
FC protocol for CL Plus 405 CD41 antibody CL405-65173Download protocol
Standard Protocols
Click here to view our Standard Protocols