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验证数据展示

  • FC experiment of human PBMCs using 98134-1-RR

    FC experiment of human PBMCs using 98134-1-RR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD2 Rabbit RecAb (98134-1-RR, Clone: 241222E2) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with CoraLite® Plus 647 Anti-Human CD3 (OKT3) Mouse IgG2a Recombinant Antibody (CL647-65569, Clone: OKT3). Cells were not fixed. Lymphocytes were gated.

  • FC experiment of human PBMCs using 98134-1-RR

    FC experiment of human PBMCs using 98134-1-RR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD2 Rabbit RecAb (98134-1-RR, Clone: 241222E2) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed. Lymphocytes were gated.

  • Affinity and Kinetic Characterization of 98134-1-RR

    Affinity and Kinetic Characterization of 98134-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98134-1-RR against Human CD2 were performed. The affinity constant is 0.418 nM.

Anti-Human CD2 Rabbit Recombinant Antibody

CD2 Uni-rAb® Recombinant Antibody for FC
Cat No. 98134-1-RR

产品说明书

CloneNo. 241222E2

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

FC

别名

241222E2, CD2 molecule, Erythrocyte receptor, LFA 2, LFA 3 receptor

缓冲液配方:  PBS, Azide
PBS, Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
Biotin
规格价格库存

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经过测试的应用

Positive FC detected inhuman PBMCs

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC)FC : 0.25 ug per 10^6 cells in a 100 µl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

98134-1-RR targets CD2 in FC applications and shows reactivity with human samples.

经测试应用 FC Application Description
经测试反应性 human
免疫原

CatNo: Eg1271

Product name: Recombinant Human CD2 protein (His Tag)

Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

Tag: C-6*HIS

Domain: 25-209 aa of BC033583

Sequence: KEITNALETWGALGQDINLDIPSFQMSDDIDDIKWEKTSDKKKIAQFRKEKETFKEKDTYKLFKNGTLKIKHLKTDDQDIYKVSIYDTKGKNVLEKIFDLKIQERVSKPKISWTCINTTLTCEVMNGTDPELNLYQDGKHLKLSQRVITHKWTTSLSAKFKCTAGNKVSKESSVEPVSCPEKGLD

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 CD2 molecule
别名 241222E2, CD2 molecule, Erythrocyte receptor, LFA 2, LFA 3 receptor
计算分子量 351 aa, 39 kDa
GenBank蛋白编号BC033583
基因名称 CD2
Gene ID (NCBI) 914
ENSEMBL Gene IDENSG00000116824
RRIDAB_3672280
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDP06729
储存缓冲液 PBS with 0.09% sodium azide, pH 7.3.
储存条件Store at 2 - 8°C. Stable for one year after shipment.

背景介绍

CD2 is a cell surface glycoprotein present on a majority of thymocytes, all mature T cells and subset of NK cells but not on B lymphocytes. It is a pan T-cell marker. CD2 interacts with lymphocyte function-associated antigen (LFA-3/CD58) and CD48/BCM1 to mediate adhesion between T-cells and other cell types. CD2 is implicated in the triggering of T-cells, the cytoplasmic domain is implicated in the signaling function.

实验方案

Product Specific Protocols
FC protocol for CD2 antibody 98134-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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