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验证数据展示

  • WB analysis using 89091-1-RR (same clone as 89091-1-PBS)

    WB analysis using 89091-1-RR (same clone as 89091-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 89091-1-RR (EIF4E antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 89091-1-PBS in a different storage buffer formulation.

  • WB analysis of HSC-T6 using 89091-1-RR (same clone as 89091-1-PBS)

    WB analysis of HSC-T6 using 89091-1-RR (same clone as 89091-1-PBS)

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 89091-1-RR (EIF4E antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 89091-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 89091-1-RR (same clone as 89091-1-PBS)

    IF Staining of HepG2 using 89091-1-RR (same clone as 89091-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using EIF4E antibody (89091-1-RR, Clone: 262243C4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 89091-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 89091-1-RR (same clone as 89091-1-PBS)

    FC experiment of HeLa using 89091-1-RR (same clone as 89091-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug EIF4E Recombinant monoclonal antibody (89091-1-RR, Clone:262243C4)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Intracellular Flow Cytometry Fixation & Permeabilization Buffer Kit (PF00019). This data was developed using the same antibody clone with 89091-1-PBS in a different storage buffer formulation.

  • Cytometric bead array sample test of MP03801-1

    Cytometric bead array sample test of MP03801-1

    The mean EIF4E concentration was determined to be 316 ng/mL in Ramos cell extract based on a 1.2 mg/mL extract load, 347.5 ng/mL in Raji cell extract based on a 1.2 mg/mL extract load, 130.6 ng/mL in 5637 cell extract based on a 1.2 mg/mL extract load.

  • Cytometric bead array standard curve of MP03801-1

    Cytometric bead array standard curve of MP03801-1

    Cytometric bead array standard curve of MP03801-1, EIF4E Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 89091-1-PBS. Detection antibody: 89091-3-PBS. Standard: Ag27191. Range: 0.312-40 ng/mL.

  • Affinity of 89091-1-RR

    Affinity of 89091-1-RR

    Biolayer interferometry (BLI) kinetic assays of 89091-1-RR against Human EIF4E were performed. The affinity constant is below 1 pM.

EIF4E Recombinant monoclonal antibody, PBS Only (Capture)

EIF4E Uni-rAb® Recombinant Antibody for WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA
Cat No. 89091-1-PBS

产品说明书

CloneNo. 262243C4

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

别名

eIF 4E, eIF-4E, EIF4EL1, EIF4F, eIF-4F 25 kDa subunit

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy EIF4E Ready-To-Use IHC Kit. EIF4E primary antibody included.

产品信息

89091-1-PBS targets EIF4E as part of a matched antibody pair:

MP03801-1: 89091-1-PBS capture and 89091-3-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag27191

Product name: Recombinant human EIF4E protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-217 aa of BC012611

Sequence: MATVEPETTPTPNPPTTEEEKTESNQEVANPEHYIKHPLQNRWALWFFKNDKSKTWQANLRLISKFDTVEDFWALYNHIQLSSNLMPGCDYSLFKDGIEPMWEDEKNKRGGRWLITLNKQQRRSDLNRFWLETLLCLIGESFDDYSDDVCGAVVNVRAKGDKIAIWTTECENREAVTHIGRVYKERLGLPPKIVIGYQSHADTATKSGSTTKNRFVV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 eukaryotic translation initiation factor 4E
别名 eIF 4E, eIF-4E, EIF4EL1, EIF4F, eIF-4F 25 kDa subunit
计算分子量 29 kDa
观测分子量 26-29 kDa
GenBank蛋白编号BC012611
基因名称 EIF4E
Gene ID (NCBI) 1977
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP06730
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Eukaryotic translation initiation factor 4E, also known as eIF4E, is a protein that in humans is encoded by the EIF4E gene. eIF4E is the mRNA cap-binding protein, known as a general initiation factor allowing for mRNA-ribosome interaction and cap-dependent translation in eukaryotic cells. eIF4E is a polypeptide that exists as both a free form and as part of the eIF4F pre-initiation complex. Regulation of eIF4E may be achieved via three distinct mechanisms: transcription, phosphorylation, and inhibitory proteins. This is a rabbit polyclonal antibody raised against the full-length human EIF4E.

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