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验证数据展示

  • WB analysis using 88895-2-RR

    WB analysis using 88895-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 88895-2-RR (JUP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis using 88895-2-RR

    WB analysis using 88895-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 88895-2-RR (JUP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis using 88895-2-RR

    WB analysis using 88895-2-RR

    C2C12 cells lysates were subjected to SDS PAGE followed by western blot with 88895-2-RR (JUP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of HT-29 using 88895-2-RR

    IF Staining of HT-29 using 88895-2-RR

    Immunofluorescent analysis of (4% PFA) fixed HT-29 cells using Gamma Catenin antibody (88895-2-RR, Clone: 261780E6 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Gamma Catenin Recombinant monoclonal antibody

Gamma Catenin Uni-rAb® Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 88895-2-RR

产品说明书

CloneNo. 261780E6

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, canine

应用

WB, IF/ICC, ELISA

别名

JUP, JUP/gamma Catenin, γ-Catenin, ARVD12, Catenin gamma

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHEK-293 cells, MDCK cells, C2C12 cells, A431 cells, MCF-7 cells, MDA-MB-231 cells, mouse heart tissue
Positive IF/ICC detected inHT-29 cells
Planning an IHC experiment? We recommend our IHCeasy JUP Ready-To-Use IHC Kit. JUP primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

88895-2-RR targets Gamma Catenin in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, canine samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, canine
免疫原

CatNo: Ag1627

Product name: Recombinant human JUP protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 396-745 aa of BC011865

Sequence: LVNQLSVDDVNVLTCATGTLSNLTCNNSKNKTLVTQNSGVEALIHAILRAGDKDDITEPAVCALRHLTSRHPEAEMAQNSVRLNYGIPAIVKLLNQPNQWPLVKATIGLIRNLALCPANHAPLQEAAVIPRLVQLLVKAHQDAQRHVAAGTQQPYTDGVRMEEIVEGCTGALHILARDPMNRMEIFRLNTIPLFVQLLYSSVENIQRVAAGVLCELAQDKEAADAIDAEGASAPLMELLHSRNEGTATYAAAVLFRISEDKNPDYRKRVSVELTNSLFKHDPAAWEAAQSMIPINEPYGDDLDATYRPMYSSDVPLDPLEMHMDMDGDYPIDTYSDGLRPPYPTADHMLA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 junction plakoglobin
别名 JUP, JUP/gamma Catenin, γ-Catenin, ARVD12, Catenin gamma
计算分子量 82 kDa
观测分子量82 kDa
GenBank蛋白编号BC011865
基因名称 Gamma Catenin
Gene ID (NCBI) 3728
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP14923
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Plakoglobin (γ-catenin), a member of the Armadillo proteins, plays important roles in cell adhesion with α-catenin and β-catenin, is involved in Wnt signaling, and is translocated into the nucleus and binds LEF1. Plakoglobin is a major cytoplasmic component of both desmosomes and adherens junctions, and is the only known constituent common to submembranous plaques in both of these structures, which are located at the intercalated disc (ICD) of cardiomyocytes. Plakoglobin links cadherins to the actin cytoskeleton. Mutations in plakoglobin are associated with arrhythmogenic right ventricular dysplasia and Pemphigus vulgaris.

实验方案

Product Specific Protocols
IF protocol for Gamma Catenin antibody 88895-2-RRDownload protocol
WB protocol for Gamma Catenin antibody 88895-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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