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验证数据展示

  • WB analysis using 88823-1-RR (same clone as 88823-1-PBS)

    WB analysis using 88823-1-RR (same clone as 88823-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 88823-1-RR (P27; KIP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 88823-1-PBS in a different storage buffer formulation.

  • IF Staining of NIH/3T3 using 88823-1-RR (same clone as 88823-1-PBS)

    IF Staining of NIH/3T3 using 88823-1-RR (same clone as 88823-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using P27; KIP1 antibody (88823-1-RR, Clone: 261313A2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 88823-1-PBS in a different storage buffer formulation.

  • IP experiment of NIH/3T3 using 88823-1-RR (same clone as 88823-1-PBS)

    IP experiment of NIH/3T3 using 88823-1-RR (same clone as 88823-1-PBS)

    IP result of anti-P27; KIP1 (IP:88823-1-RR, 4ug; Detection:88823-1-RR 1:1500) with NIH/3T3 cells lysate 1040 ug. This data was developed using the same antibody clone with 88823-1-PBS in a different storage buffer formulation.

  • IHC staining of human ovary cancer using 88823-1-RR (same clone as 88823-1-PBS)

    IHC staining of human ovary cancer using 88823-1-RR (same clone as 88823-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 88823-1-RR (P27; KIP1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 88823-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CDKN1B antibody included
  • IHC staining of human ovary cancer using 88823-1-RR (same clone as 88823-1-PBS)

    IHC staining of human ovary cancer using 88823-1-RR (same clone as 88823-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 88823-1-RR (P27; KIP1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 88823-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CDKN1B antibody included
  • Cytometric bead array sample test of MP03680-1

    Cytometric bead array sample test of MP03680-1

    The mean P27; KIP1 concentration was determined to be 6.5 ng/mL in MCF-7 cell extract based on a 1.2 mg/mL extract load, 0.4 ng/mL in PC-3 cell extract based on a 1.2 mg/mL extract load.

  • Cytometric bead array standard curve of MP03680-1

    Cytometric bead array standard curve of MP03680-1

    Cytometric bead array standard curve of MP03680-1, P27; KIP1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 88823-1-PBS. Detection antibody: 88823-2-PBS. Standard: Ag22582. Range: 0.312-40 ng/mL.

  • Affinity of 88823-1-RR

    Affinity of 88823-1-RR

    Biolayer interferometry (BLI) kinetic assays of 88823-1-RR against Human P27; KIP1 were performed. The affinity constant is 0.576 nM.

P27; KIP1 Recombinant monoclonal antibody, PBS Only (Capture)

P27; KIP1 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, IP, Cytometric bead array, Indirect ELISA
Cat No. 88823-1-PBS

产品说明书

CloneNo. 261313A2

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, Cytometric bead array, Indirect ELISA

别名

CDKN1B, P27, CDKN4, Cyclin-dependent kinase inhibitor 1B, Cyclin-dependent kinase inhibitor p27

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

点击咨询售前客服

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Planning an IHC experiment? We recommend our IHCeasy CDKN1B Ready-To-Use IHC Kit. CDKN1B primary antibody included.

产品信息

88823-1-PBS targets P27; KIP1 as part of a matched antibody pair:

MP03680-1: 88823-1-PBS capture and 88823-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, IF/ICC, IP, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag22582

Product name: Recombinant human P27; KIP1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 44-198 aa of BC001971

Sequence: DLEKHCRDMEEASQRKWNFDFQNHKPLEGKYEWQEVEKGSLPEFYYRPPRPPKGACKVPAQESQDGSGSRPAAPLIGAPANSEDTHLVDPKTDPSDSQTGLAEQCAGIRKRPATDDSSTQNKRANRTEENVSDGSPNAGSVEQTPKKPGLRRRQT

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 cyclin-dependent kinase inhibitor 1B (p27, Kip1)
别名 CDKN1B, P27, CDKN4, Cyclin-dependent kinase inhibitor 1B, Cyclin-dependent kinase inhibitor p27
计算分子量 198 aa, 22 kDa
观测分子量27 kDa
GenBank蛋白编号BC001971
基因名称 p27 Kip1/CDKN1B
Gene ID (NCBI) 1027
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP46527
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CDKN1B, also named as P27 or KIP1, is a cyclin-dependent kinase inhibitor, which shares a limited similarity with CDK inhibitor CDKN1A/p21. P27 binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controlling cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state. Downregulation of P27 has been implicated in the progression of several malignancies, including lung cancer, hepatocellular carcinoma, salivary cancer, oral squamous cell carcinomas, and gastric cancer.

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