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验证数据展示

  • WB analysis using 88812-2-RR

    WB analysis using 88812-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 88812-2-RR (TK1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human tonsillitis using 88812-2-RR

    IHC staining of human tonsillitis using 88812-2-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 88812-2-RR (TK1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TK1 antibody included
  • IHC staining of human tonsillitis using 88812-2-RR

    IHC staining of human tonsillitis using 88812-2-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 88812-2-RR (TK1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TK1 antibody included
  • Affinity of 88812-2-RR

    Affinity of 88812-2-RR

    Biolayer interferometry (BLI) kinetic assays of 88812-2-RR against Human TK1 were performed. The affinity constant is below 1 pM.

TK1 Recombinant monoclonal antibody

TK1 Uni-rAb® Recombinant Antibody for WB, IHC, ELISA
Cat No. 88812-2-RR

产品说明书

CloneNo. 261632C8

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

EC:2.7.1.21, TK 1, TK 2, Tk1a, Tk1b

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHEK-293 cells, MCF-7 cells, Jurkat cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy TK1 Ready-To-Use IHC Kit. TK1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

88812-2-RR targets TK1 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag8249

Product name: Recombinant human TK1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-234 aa of BC007986

Sequence: MSCINLPTVLPGSPSKTRGQIQVILGPMFSGKSTELMRRVRRFQIAQYKCLVIKYAKDTRYSSSFCTHDRNTMEALPACLLRDVAQEALGVAVIGIDEGQFFPDIVEFCEAMANAGKTVIVAALDGTFQRKPFGAILNLVPLAESVVKLTAVCMECFREAAYTKRLGTEKEVEVIGGADKYHSVCRLCYFKKASGQPAGPDNKENCPVPGKPGEAVAARKLFAPQQILQCSPAN

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 thymidine kinase 1, soluble
别名 EC:2.7.1.21, TK 1, TK 2, Tk1a, Tk1b
计算分子量 25 kDa
观测分子量 25-30 kDa
GenBank蛋白编号BC007986
基因名称 TK1
Gene ID (NCBI) 7083
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP04183
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TK1(Thymidine kinase, cytosolic) belongs to the thymidine kinase family and is a regulatory enzyme that can interchange between its dimeric and tetrameric forms, which have low and high catalytic efficiencies, respectively. TK1 is involved in DNA synthesis and an important proliferation marker(PMID:21178264).

实验方案

Product Specific Protocols
IHC protocol for TK1 antibody 88812-2-RRDownload protocol
WB protocol for TK1 antibody 88812-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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