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验证数据展示

  • WB analysis using 88414-1-RR (same clone as 88414-1-PBS)

    WB analysis using 88414-1-RR (same clone as 88414-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 88414-1-RR (ATXN10 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 88414-1-PBS in a different storage buffer formulation.

  • WB analysis using 88414-1-RR (same clone as 88414-1-PBS)

    WB analysis using 88414-1-RR (same clone as 88414-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 88414-1-RR (ATXN10 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 88414-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA sample test of MP03536-2

    Sandwich ELISA sample test of MP03536-2

    The mean ATXN10 concentration was determined to be 81.1 ng/mL in A431 cell extract based on a 1.2 mg/mL extract load, 17.3 ng/mL in U-251 cell extract based on a 1.5 mg/mL extract load and 18.6 ng/mL in U2OS cell extract based on a 1.2 mg/mL extract load.

  • Sandwich ELISA standard curve of MP03536-2

    Sandwich ELISA standard curve of MP03536-2

    Sandwich ELISA standard curve of MP03536-2, Human ATXN10 Recombinant Matched Antibody Pair - PBS only. 88414-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag8259. 88414-3-PBS was HRP conjugated as the detection antibody. Range: 39.1-2500 pg/mL.

  • Cytometric bead array sample test of MP03536-1

    Cytometric bead array sample test of MP03536-1

    The mean ATXN10 concentration was determined to be 80.7 ng/mL in HeLa cell extract based on a 1.2 mg/mL extract load, 13.9 ng/mL in U2OS cell extract based on a 1.2 mg/mL extract load.

  • Cytometric bead array standard curve of MP03536-1

    Cytometric bead array standard curve of MP03536-1

    Cytometric bead array standard curve of MP03536-1, ATXN10 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 88414-1-PBS. Detection antibody: 88414-2-PBS. Standard: Ag8259. Range: 0.156-20 ng/mL.

ATXN10 Recombinant monoclonal antibody, PBS Only (Capture)

ATXN10 Uni-rAb® Recombinant Antibody for WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA
Cat No. 88414-1-PBS

产品说明书

CloneNo. 260934C7

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA

别名

ataxin 10, Ataxin-10, E46L, SCA10

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

88414-1-PBS targets ATXN10 as part of a matched antibody pair:

MP03536-1: 88414-1-PBS capture and 88414-2-PBS detection (validated in Cytometric bead array)

MP03536-2: 88414-1-PBS capture and 88414-3-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag8259

Product name: Recombinant human ATXN10 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 120-475 aa of BC007508

Sequence: ELRVEQESLLTAFRCGLQFLGNIASRNEDSQSIVWVHAFPELFLSCLNHPDKKIVAYSSMILFTSLNHERMKELEENLNIAIDVIDAYQKHPESEWPFLIITDLFLKSPELVQAMFPKLNNQERVTLLDLMIAKITSDEPLTKDDIPVFLRHAELIASTFVDQCKTVLKLASEEPPDDEEALATIRLLDVLCEMTVNTELLGYLQVFPGLLERVIDLLRVIHVAGKETTNIFSNCGCVRAEGDISNVANGFKSHLIRLIGNLCYKNKDNQDKVNELDGIPLILDNCNISDSNPFLTQWVIYAIRNLTEDNSQNQDLIAKMEEQGLADASLLKKVGFEVEKKGEKLILKSTRDTPKP

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 ataxin 10
别名 ataxin 10, Ataxin-10, E46L, SCA10
计算分子量 53 kDa
观测分子量45-50 kDa
GenBank蛋白编号BC007508
基因名称 ATXN10
Gene ID (NCBI) 25814
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9UBB4
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ATXN10, also named as SCA10, is a 475 amino acid protein, which belongs to the ataxin-10 family. ATXN10 is expressed in the central nervous system.ATXN10 is necessary for the survival of cerebellar neurons. ATXN10 may play a role in the maintenance of a critical intracellular glycosylation level and homeostasis.

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