The mean PIK3C2A concentration was determined to be 11.0 ng/mL in HaCaT cell extract based on a 1.2 mg/mL extract load, 9.6 ng/mL in PC-3 cell extract based on a 1.2 mg/mL extract load and 3.4 ng/mL in HT-1080 cell extract based on a 1.2 mg/mL extract load.

The mean PIK3C2A concentration was determined to be 3.1 ng/mL in THP-1 cell extract based on a 1.2 mg/mL extract load, 4.0 ng/mL in MG-63 cell extract based on a 1.2 mg/mL extract load and 3.4 ng/mL in HL-60 cell extract based on a 1.2 mg/mL extract load.

The mean PIK3C2A concentration was determined to be 16.3 ng/mL in HT-29 cell extract based on a 1.2 mg/mL extract load, 7.3 ng/mL in A431 cell extract based on a 1.2 mg/mL extract load and 10.5 ng/mL in RT-4 cell extract based on a 1.2 mg/mL extract load.

Sandwich ELISA standard curve of MP03538-1, Human PIK3C2A Recombinant Matched Antibody Pair - PBS only. 88380-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag16894. 88380-3-PBS was HRP conjugated as the detection antibody. Range: 39.1-2500 pg/mL.

The mean PIK3C2A concentration was determined to be 10.2 ng/mL in Caco-2 cell extract based on a 1.2 mg/mL extract load, 15.5 ng/mL in HT-29 cell extract based on a 1.2 mg/mL extract load, 3.0 ng/mL in HL-60 cell extract based on a 1.2 mg/mL extract load.

Cytometric bead array standard curve of MP03538-1, PIK3C2A Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 88380-1-PBS. Detection antibody: 88380-3-PBS. Standard: Ag16894. Range: 0.156-20 ng/mL.