验证数据展示
产品信息
88290-1-PBS targets NECAB2 as part of a matched antibody pair:
MP03437-1: 88290-2-PBS capture and 88290-1-PBS detection (validated in Cytometric bead array)
Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.
This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.
| 经测试应用 | WB, Cytometric bead array, Indirect ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag2909 Product name: Recombinant human NECAB2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 125-386 aa of BC016979 Sequence: DHMGDYEDVLASLETLNHSVLKAMGYTKKVYEGGSNVDQFVTRFLLKETANQIQSLLSSVESAVEAIEEQTSQLRQNHIKPSHSAAQTWCGSPTPASAPNHKLMAMEQGKTLPSATEDAKEEGLEAQISRLAELIGRLESKALWFDLQQRLSDEDGTNMHLQLVRQEMAVCPEQLSEFLDSLRQYLRGTTGVRNCFHITAVRLSDGFTFVIYEFWETEEAWKRHLQSPLCKAFRHVKVDTLSQPEALSRILVPAAWCTVGRD 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | N-terminal EF-hand calcium binding protein 2 |
| 别名 | |
| 计算分子量 | 386 aa, 42 kDa |
| 观测分子量 | 37 kDa |
| GenBank蛋白编号 | BC016979 |
| 基因名称 | NECAB2 |
| Gene ID (NCBI) | 54550 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q7Z6G3 |
| 储存缓冲液 | PBS only, pH 7.3. |
| 储存条件 | Store at -80°C. The product is shipped with ice packs. Upon receipt, store it immediately at -80°C |
背景介绍
NECAB2 (N-terminal EF-hand calcium-binding protein 2) is a neuronal calcium sensor that functions as a signaling scaffold to modulate G protein-coupled receptor activity. It binds calcium via its N-terminal EF-hand domain and interacts directly with the adenosine A2A receptor (ADORA2A) and metabotropic glutamate receptor 5 (mGluR5), regulating their cell surface expression, internalization, and downstream signaling such as ERK1/2 activation . The protein is predominantly expressed in the brain and spinal cord, where it plays a critical role in pain signaling; its loss in dorsal root ganglia and spinal interneurons significantly accelerates recovery from inflammatory pain hypersensitivity in mice.
