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验证数据展示

  • WB analysis using 88167-2-RR (same clone as 88167-2-PBS)

    WB analysis using 88167-2-RR (same clone as 88167-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 88167-2-RR (G6B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 88167-2-PBS in a different storage buffer formulation.

  • Sandwich ELISA sample test of MP03352-2

    Sandwich ELISA sample test of MP03352-2

    The mean MPIG6B concentration was determined to be 2.2 ng/mL in K-562 cell extract based on a 1.2 mg/mL extract load, 5.8 ng/mL in HEL cell extract based on a 1.5 mg/mL extract load, 1.1 ng/mL in 786-O cell extract based on a 1.2 mg/mL extract load, 2.4 ng/mL in THP-1 cell extract based on a 1.5 mg/mL extract load and 1.1 ng/mL in RT-4 cell extract based on a 1.5 mg/mL extract load.

  • Sandwich ELISA standard curve of MP03352-2

    Sandwich ELISA standard curve of MP03352-2

    Sandwich ELISA standard curve of MP03352-2, Human MPIG6B Recombinant Matched Antibody Pair - PBS only. 88167-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg5762. 88167-3-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/mL.

  • IP experiment of human peripheral blood platelets using 88167-2-RR (same clone as 88167-2-PBS)

    IP experiment of human peripheral blood platelets using 88167-2-RR (same clone as 88167-2-PBS)

    IP result of anti-MPIG6B (IP:88167-2-RR, 4ug; Detection:88167-2-RR 1:3000) with human peripheral blood platelets lysate 880 ug. This data was developed using the same antibody clone with 88167-2-PBS in a different storage buffer formulation.

  • Cytometric bead array sample test of MP03352-1

    Cytometric bead array sample test of MP03352-1

    The mean MPIG6B concentration was determined to be 8.8 ng/mL in HEL cell extract based on a 1.5 mg/mL extract load, THP-1 cell extract were served as a negative control.

  • Cytometric bead array standard curve of MP03352-1

    Cytometric bead array standard curve of MP03352-1

    Cytometric bead array standard curve of MP03352-1, MPIG6B Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 88167-2-PBS. Detection antibody: 88167-1-PBS. Standard: Eg5762. Range: 0.156-20 ng/mL.

MPIG6B Recombinant monoclonal antibody, PBS Only (Capture)

MPIG6B Uni-rAb® Recombinant Antibody for WB, IP, Cytometric bead array, Sandwich ELISA, Indirect ELISA
Cat No. 88167-2-PBS

产品说明书

CloneNo. 260393F2

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IP, Cytometric bead array, Sandwich ELISA, Indirect ELISA

别名

C6orf25, G6B, G6B-B, Megakaryocyte and platelet inhibitory receptor G6b, NG31

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

88167-2-PBS targets MPIG6B as part of a matched antibody pair:

MP03352-1: 88167-2-PBS capture and 88167-1-PBS detection (validated in Cytometric bead array)

MP03352-2: 88167-2-PBS capture and 88167-3-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IP, Cytometric bead array, Sandwich ELISA, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Eg5762

Product name: Recombinant human G6B protein

Source: mammalian cells-derived, V37

Tag: C-rFc

Domain: 18-142 aa of NM_138272.3

Sequence: NPGASLDGRPGDRVNLSCGGVSHPIRWVWAPSFPACKGLSKGRRPILWASSSGTPTVPPLQPFVGRLRSLDSGIRRLELLLSAGDSGTFFCKGRHEDESRTVLHVLGDRTYCKAPGPTHGSVYPQ

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 chromosome 6 open reading frame 25
别名 C6orf25, G6B, G6B-B, Megakaryocyte and platelet inhibitory receptor G6b, NG31
计算分子量26 kDa
观测分子量17kDa, 26 kDa
GenBank蛋白编号NM_138272.3
基因名称 C6orf25
Gene ID (NCBI) 80739
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDO95866-1
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MPIG6B (Megakaryocyte and Platelet Inhibitory Receptor G6b), also known as G6b-B or C6orf25, is a type I transmembrane glycoprotein belonging to the immunoglobulin superfamily, and serves as a key negative regulatory receptor for platelet activation and megakaryocyte differentiation. The intracellular domain of MPIG6B contains immunoreceptor tyrosine-based inhibitory motifs (ITIMs), which recruit SHP-1/SHP-2 phosphatases to inhibit Src family kinase activity, thereby negatively regulating platelet aggregation, release reactions, and megakaryocyte maturation. Mutations in the MPIG6B gene lead to thrombocytopenia and bleeding tendency, and its functional dysregulation is associated with immune thrombocytopenia (ITP) and myeloproliferative neoplasms.

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