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验证数据展示

  • WB analysis using 88052-1-RR (same clone as 88052-1-PBS)

    WB analysis using 88052-1-RR (same clone as 88052-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 88052-1-RR (SUB1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 88052-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA sample test of MP03355-2

    Sandwich ELISA sample test of MP03355-2

    The mean SUB1 concentration was determined to be 523.40 ng/mL in HL-60 cell extract based on a 1.20 mg/mL extract load and 122.10 ng/mL in Ramos cell extract based on a 1.50 mg/mL extract load.

  • Sandwich ELISA standard curve of MP03355-2

    Sandwich ELISA standard curve of MP03355-2

    Sandwich ELISA standard curve of MP03355-2, Human SUB1 Recombinant Matched Antibody Pair - PBS only. 88052-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag1390. 88052-1-PBS was HRP conjugated as the detection antibody. Range: 0.625-20 ng/mL.

SUB1 Recombinant monoclonal antibody, PBS Only (Detector)

SUB1 Uni-rAb® Recombinant Antibody for WB, Sandwich ELISA, Indirect ELISA
Cat No. 88052-1-PBS

产品说明书

CloneNo. 260206C11

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, Sandwich ELISA, Indirect ELISA

别名

Activated RNA polymerase II transcriptional coactivator p15, p14, P15, PC4, Positive cofactor 4

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy SUB1 Ready-To-Use IHC Kit. SUB1 primary antibody included.

产品信息

88052-1-PBS targets SUB1 as part of a matched antibody pair:

MP03355-2: 88052-3-PBS capture and 88052-1-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, Sandwich ELISA, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag1390

Product name: Recombinant human SUB1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-127 aa of BC009610

Sequence: MPKSKELVSSGSSGSDSDSEVDKKLKRKKQVAPEKPVKKQKTGETSRALSSSKQSSSSRDDNMFQIGKMRYVSVRDFKGKVLIDIREYWMDPEGEMKPGRKGISLNPEQWSQLKEQISDIDDAVRKL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 SUB1 homolog (S. cerevisiae)
别名 Activated RNA polymerase II transcriptional coactivator p15, p14, P15, PC4, Positive cofactor 4
计算分子量 14 kDa
观测分子量18-20 kDa
GenBank蛋白编号BC009610
基因名称 SUB1
Gene ID (NCBI) 10923
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP53999
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SUB1 gene encodes activated RNA polymerase II transcriptional coactivator p15, also termed as positive cofactor 4 (PC4) or p14. SUB1 is a sort of general coactivator that functions in assembling and stablization of transcription complex. It has DNA binding activity and is localized in the nucleus, mulitple modification sites of phosphorylation or acetylation may turn it appear slightly shifted molecular weight.

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