The mean Gamma Tubulin concentration was determined to be 49.70 ng/mL in Ramos cell extract based on a 1.20 mg/mL extract load, 78.29 ng/mL in HepG2 cell extract based on a 1.20 mg/mL extract load, 50.94 ng/mL in HL-60 cell extract based on a 1.50 mg/mL extract load, 35.84 ng/mL in Daudi cell extract based on a 1.20 mg/mL extract load, 55.86 ng/mL in HeLa cell extract based on a 1.20 mg/mL extract load, 77.92 ng/mL in Jurkat cell extract based on a 1.20 mg/mL extract load and 57.91 ng/mL in HEK-293 cell extract based on a 1.50 mg/mL extract load.

Sandwich ELISA standard curve of MP03327-1, Human Gamma Tubulin Recombinant Matched Antibody Pair - PBS only. 87860-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag7070. 87860-3-PBS was HRP conjugated as the detection antibody. Range: 0.195-12.5 ng/mL.