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验证数据展示

  • WB analysis of HEK-293 using 87859-2-RR (same clone as 87859-2-PBS)

    WB analysis of HEK-293 using 87859-2-RR (same clone as 87859-2-PBS)

    WB result of AP1B1 antibody (87859-2-RR; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-AP1B1 transfected HEK-293 cells. This data was developed using the same antibody clone with 87859-2-PBS in a different storage buffer formulation.

  • WB analysis using 87859-2-RR (same clone as 87859-2-PBS)

    WB analysis using 87859-2-RR (same clone as 87859-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 87859-2-RR (AP1B1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87859-2-PBS in a different storage buffer formulation.

  • IP experiment of HEK-293 using 87859-2-RR (same clone as 87859-2-PBS)

    IP experiment of HEK-293 using 87859-2-RR (same clone as 87859-2-PBS)

    IP result of anti-AP1B1 (IP:87859-2-RR, 4ug; Detection:87859-2-RR 1:3000) with HEK-293 cells lysate 1560 ug. This data was developed using the same antibody clone with 87859-2-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 87859-2-RR (same clone as 87859-2-PBS)

    IF Staining of HepG2 using 87859-2-RR (same clone as 87859-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using AP1B1 antibody (87859-2-RR, Clone: 253524D8 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 87859-2-PBS in a different storage buffer formulation.

  • Cytometric bead array sample test of MP03244-1

    Cytometric bead array sample test of MP03244-1

    The mean AP1B1 concentration was determined to be 118.8 ng/mL in A431 cell extract based on a 1.2 mg/mL extract load, 163.8 ng/mL in HEK-293 cell extract based on a 1.5 mg/mL extract load, 80.2 ng/mL in A2780 cell extract based on a 1.2 mg/mL extract load.

  • Cytometric bead array standard curve of MP03244-1

    Cytometric bead array standard curve of MP03244-1

    Cytometric bead array standard curve of MP03244-1, AP1B1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 87859-3-PBS. Detection antibody: 87859-2-PBS. Standard: Ag10417. Range: 0.312-40 ng/mL.

  • Affinity of 87859-2-RR

    Affinity of 87859-2-RR

    Biolayer interferometry (BLI) kinetic assays of 87859-2-RR against Human AP1B1 were performed. The affinity constant is 0.356 nM.

AP1B1 Recombinant monoclonal antibody, PBS Only (Detector)

AP1B1 Uni-rAb® Recombinant Antibody for WB, IF/ICC, IP, Cytometric bead array, Indirect ELISA
Cat No. 87859-2-PBS

产品说明书

CloneNo. 253524D8

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, IP, Cytometric bead array, Indirect ELISA

别名

Adaptor protein complex AP-1 subunit beta-1, Adaptor-related protein complex 1 subunit beta-1, ADTB1, AP 1 complex subunit beta 1, AP-1 complex subunit beta-1

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

87859-2-PBS targets AP1B1 as part of a matched antibody pair:

MP03244-1: 87859-3-PBS capture and 87859-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IF/ICC, IP, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag10417

Product name: Recombinant human AP1B1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 671-919 aa of BC046242

Sequence: NFVAPPTAAVPANLGAPIGSGLSDLFDLTSGVGTLSGSYVAPKAVGSISMDLQLTNKALQVMTDFAIQFNRNSFGLAPAAPLQVHAPLSPNQTVEISLPLSTVGSVMKMEPLNNLQVAVKNNIDVFYFSTLYPLHILFVEDGKMDRQMFLATWKDIPNENEAQFQIRDCPLNAEAASSKLQSSNIFTVAKRNVEGQDMLYQSLKLTNGIWVLAELRIQPGNPSCTLSLKCRAPEVSQHVYQAYETILKN

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 adaptor-related protein complex 1, beta 1 subunit
别名 Adaptor protein complex AP-1 subunit beta-1, Adaptor-related protein complex 1 subunit beta-1, ADTB1, AP 1 complex subunit beta 1, AP-1 complex subunit beta-1
计算分子量 919 aa, 101 kDa
观测分子量 101 kDa
GenBank蛋白编号BC046242
基因名称 AP1B1
Gene ID (NCBI) 162
RRIDAB_3745793
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ10567
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

AP1B1 is a subunit of the adaptor complex AP-1 and is a member of the adaptin protein family. Adaptor protein (AP) complexes are cytosolic heterotetramers that mediate the sorting of membrane proteins in the secretory and endocytic pathways. AP-1 is found at the cytoplasmic face of coated vesicles located at the Golgi complex, where it mediates both the recruitment of clathrin to the membrane and the recognition of sorting signals within the cytosolic tails of transmembrane receptors.

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