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验证数据展示

  • WB analysis using 87782-2-RR (same clone as 87782-2-PBS)

    WB analysis using 87782-2-RR (same clone as 87782-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 87782-2-RR (Claudin 1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87782-2-PBS in a different storage buffer formulation.

  • WB analysis of HT-29 using 87782-2-RR (same clone as 87782-2-PBS)

    WB analysis of HT-29 using 87782-2-RR (same clone as 87782-2-PBS)

    HT-29 cells were subjected to SDS PAGE followed by western blot with 87782-2-RR (Claudin 1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87782-2-PBS in a different storage buffer formulation.

  • WB analysis using 87782-2-RR (same clone as 87782-2-PBS)

    WB analysis using 87782-2-RR (same clone as 87782-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 87782-2-RR (Claudin 1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87782-2-PBS in a different storage buffer formulation.

  • IF Staining of HaCaT using 87782-2-RR (same clone as 87782-2-PBS)

    IF Staining of HaCaT using 87782-2-RR (same clone as 87782-2-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HaCaT cells using Claudin 1 antibody (87782-2-RR, Clone: 253345A9 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 87782-2-PBS in a different storage buffer formulation.

  • Sandwich ELISA sample test of MP03201-1

    Sandwich ELISA sample test of MP03201-1

    The mean Claudin 1 concentration was determined to be 67.6 ng/mL in HUVEC cell extract based on a 1.5 mg/mL extract load and 5.0 ng/mL in MDA-MB-231 cell extract based on a 1.2 mg/mL extract load.

  • Sandwich ELISA standard curve of MP03201-1

    Sandwich ELISA standard curve of MP03201-1

    Sandwich ELISA standard curve of MP03201-1, Human Claudin 1 Recombinant Matched Antibody Pair - PBS only. 87782-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag29269. 87782-1-PBS was HRP conjugated as the detection antibody. Range: 78.1-2500 pg/mL.

  • IHC staining of human skin using 87782-2-RR (same clone as 87782-2-PBS)

    IHC staining of human skin using 87782-2-RR (same clone as 87782-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 87782-2-RR (Claudin 1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 87782-2-PBS in a different storage buffer formulation.

  • IHC staining of human skin using 87782-2-RR (same clone as 87782-2-PBS)

    IHC staining of human skin using 87782-2-RR (same clone as 87782-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 87782-2-RR (Claudin 1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 87782-2-PBS in a different storage buffer formulation.

  • IF Staining of mouse colon using 87782-2-RR (same clone as 87782-2-PBS)

    IF Staining of mouse colon using 87782-2-RR (same clone as 87782-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse colon tissue using Claudin 1 antibody (87782-2-RR, Clone: 253345A9 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 87782-2-PBS in a different storage buffer formulation.

  • IF Staining of rat colon using 87782-2-RR (same clone as 87782-2-PBS)

    IF Staining of rat colon using 87782-2-RR (same clone as 87782-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat colon tissue using Claudin 1 antibody (87782-2-RR, Clone: 253345A9 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 87782-2-PBS in a different storage buffer formulation.

  • IF Staining of HaCaT using 87782-2-RR (same clone as 87782-2-PBS)

    IF Staining of HaCaT using 87782-2-RR (same clone as 87782-2-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HaCaT cells using Claudin 1 antibody (87782-2-RR, Clone: 253345A9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 87782-2-PBS in a different storage buffer formulation.

  • Cytometric bead array sample test of MP03201-1

    Cytometric bead array sample test of MP03201-1

    The mean Claudin 1 concentration was determined to be 2.2 ng/mL in A431 cell extract based on a 1.5 mg/mL extract load, 15.2 ng/mL in A549 cell extract based on a 1.2 mg/mL extract load, HCT-116 cell extract were served as a negative control.

  • Cytometric bead array standard curve of MP03201-1

    Cytometric bead array standard curve of MP03201-1

    Cytometric bead array standard curve of MP03201-1, Claudin 1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 87782-2-PBS. Detection antibody: 87782-1-PBS. Standard: Ag29269. Range: 0.312-40 ng/mL.

Claudin 1 Recombinant monoclonal antibody, PBS Only (Capture)

Claudin 1 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, IF-P, Cytometric bead array, Sandwich ELISA, Indirect ELISA
Cat No. 87782-2-PBS

产品说明书

CloneNo. 253345A9

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IF-P, Cytometric bead array, Sandwich ELISA, Indirect ELISA

别名

Caudin 1, CLDN1, Claudin-1, CLD1, ILVASC

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

87782-2-PBS targets Claudin 1 as part of a matched antibody pair:

MP03201-1: 87782-2-PBS capture and 87782-1-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, IF/ICC, IF-P, Cytometric bead array, Sandwich ELISA, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag29269

Product name: Recombinant human Claudin 1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 102-211 aa of BC012471

Sequence: MKCMKCLEDDEVQKMRMAVIGGAIFLLAGLAILVATAWYGNRIVQEFYDPMTPVNARYEFGQALFTGWAAASLCLLGGALLCCSCPRKTTSYPTPRPYPKPAPSSGKDYV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 claudin 1
别名 Caudin 1, CLDN1, Claudin-1, CLD1, ILVASC
计算分子量 211 aa, 23 kDa
观测分子量 20-23 kDa
GenBank蛋白编号BC012471
基因名称 Claudin 1
Gene ID (NCBI) 9076
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDO95832
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Claudins are a family of proteins that are the most important components of the tight junctions, where they establish the paracellular barrier that controls the flow of molecules in the intercellular space between the cells of an epithelium. 23 Claudins have been identified. They are small (20-27 kilodaltons (kDa)) proteins with similar structures. They have four transmembrane domains, with the N-terminus and the C-terminus in the cytoplasm. Claudin-1 is an integral membrane protein expressed primarily in keratinocytes and normal mammary epithelial cells. Claudin 1 forms tight junctions with other claudin proteins and plays an important role in the intestinal epithelial barrier.

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