验证数据展示
经过测试的应用
| Positive WB detected in | A431 cells, U-87 MG cells, SH-SY5Y cells, PC-3 cells, mouse brain tissue, rat brain tissue, mouse cerebellum tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
87673-1-RR targets NCS1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Eg5439 Product name: Recombinant human NCS1 protein Source: mammalian cells-derived, V37 Tag: C-rFc Domain: 1-190 aa of NM_014286.3 Sequence: MGKSNSKLKPEVVEELTRKTYFTEKEVQQWYKGFIKDCPSGQLDAAGFQKIYKQFFPFGDPTKFATFVFNVFDENKDGRIEFSEFIQALSVTSRGTLDEKLRWAFKLYDLDNDGYITRNEMLDIVDAIYQMVGNTVELPEEENTPEKRVDRIFAMMDKNADGKLTLQEFQEGSKADPSIVQALSLYDGLV 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | frequenin homolog (Drosophila) |
| 别名 | FREQ, FLUP, Frequenin homolog, frequenin homolog (Drosophila), Frequenin like protein |
| 计算分子量 | 22 kDa |
| 观测分子量 | 20 kDa |
| GenBank蛋白编号 | NM_014286.3 |
| 基因名称 | NCS1 |
| Gene ID (NCBI) | 23413 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P62166-1 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Neuronal Calcium Sensor 1 is a member of the NCS protein family, a group of evolutionarily conserved, calcium-binding proteins predominantly expressed in the nervous system and neuroendocrine cells. NCS-1 is the vertebrate homolog of the extensively studied Drosophila protein Frequenin. It plays a crucial and multifaceted role in regulating neuronal signaling, synaptic plasticity, and neurotransmitter release.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for NCS1 antibody 87673-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
