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验证数据展示

  • WB analysis using 87655-2-RR (same clone as 87655-2-PBS)

    WB analysis using 87655-2-RR (same clone as 87655-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 87655-2-RR (RAB3D antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87655-2-PBS in a different storage buffer formulation.

  • Cytometric bead array sample test of MP03138-1

    Cytometric bead array sample test of MP03138-1

    The mean RAB3D concentration was determined to be 15.4 ng/mL in SK-BR-3 cell extract based on a 1.5 mg/mL extract load, HT-1080 cell extract were served as a negative control.

  • Cytometric bead array standard curve of MP03138-1

    Cytometric bead array standard curve of MP03138-1

    Cytometric bead array standard curve of MP03138-1, RAB3D Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 87655-2-PBS. Detection antibody: 87655-3-PBS. Standard: Eg5570. Range: 0.156-20 ng/mL.

  • Affinity of 87655-2-RR

    Affinity of 87655-2-RR

    Biolayer interferometry (BLl) kinetic assays of 87655-2-RR against Human RAB3D were performed. The affinity constant is 2.66 nM.

RAB3D Recombinant monoclonal antibody, PBS Only (Capture)

RAB3D Uni-rAb® Recombinant Antibody for WB, Cytometric bead array, Indirect ELISA
Cat No. 87655-2-PBS

产品说明书

CloneNo. 253078A7

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, Cytometric bead array, Indirect ELISA

别名

GOV, RAB16, RAD3D, Ras related protein Rab 3D, Ras-related protein Rab-3D

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

87655-2-PBS targets RAB3D as part of a matched antibody pair:

MP03138-1: 87655-2-PBS capture and 87655-3-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Eg5570

Product name: Recombinant human RAB3D protein

Source: mammalian cells-derived, V37

Tag: C-rFc

Domain: 1-219 aa of NM_004283.3

Sequence: MASAGDTQAGPRDAADQNFDYMFKLLLIGNSSVGKTSFLFRYADDSFTPAFVSTVGIDFKVKTVYRHDKRIKLQIWDTAGQERYRTITTAYYRGAMGFLLMYDIANQESFAAVQDWATQIKTYSWDNAQVILVGNKCDLEDERVVPAEDGRRLADDLGFEFFEASAKENINVKQVFERLVDVICEKMNESLEPSSSSGSNGKGPAVGDAPAPQPSSCSC

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 RAB3D, member RAS oncogene family
别名 GOV, RAB16, RAD3D, Ras related protein Rab 3D, Ras-related protein Rab-3D
计算分子量24 kDa
观测分子量 24-27 kDa
GenBank蛋白编号NM_004283.3
基因名称 RAB3D
Gene ID (NCBI) 9545
RRIDAB_3745677
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDO95716
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Rab3D is a member of the Rab3 subfamily, which comprises four isoforms (Rab3A, Rab3B, Rab3C, and Rab3D). Rab3 proteins typically associate with secretory vesicles, including synaptic vesicles, and have been implicated in the control of regulated exocytosis. Rab3D is widely expressed in adipocytes, exocrine glands and several haematopoietic cells, where it localizes to secretory granules and vesicles.

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