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验证数据展示

  • WB analysis using 87634-1-RR

    WB analysis using 87634-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 87634-1-RR (TEKT2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity of 87634-1-RR

    Affinity of 87634-1-RR

    Biolayer interferometry (BLl) kinetic assays of 87634-1-RR against Human TEKT2 were performed. The affinity constant is 8.63 nM.

TEKT2 Recombinant monoclonal antibody

TEKT2 Uni-rAb® Recombinant Antibody for WB, ELISA
Cat No. 87634-1-RR

产品说明书

CloneNo. 252977C1

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, ELISA

别名

H-tektin-T, TEKTB1, Tektin 2, Tektin B1, Tektin-2

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse testis tissue, rat testis tissue, human testis tissue, mouse ovary tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

87634-1-RR targets TEKT2 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag4457

Product name: Recombinant human TEKT2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-430 aa of BC035620

Sequence: MATLSVKPSRRFQLPDWHTNSYLLSTNAQLQRDASHQIRQEARVLRNETNNQTIWDEHDNRTRLVERIDTVNRWKEMLDKCLTDLDAEIDALTQMKESAEQNLQAKNLPLDVAIECLTLRESRRDIDVVKDPVEDELHKEVEVIEATKKALQQKVSQAFEQLCLLQEVQQQLNSDHRGKMETLEIDRGCLSLNLRSPNISLKVDPTRVPDGSTTLQQWDDFSRFNKDRAEAEMKAATELREATALTIAETNNELEAQRVATEFAFRKRLREMEKVYSELKWQEKNTLEEIAELQEDIRHLEEDLRTKLLSLKLSHTRLEARTYRPNVELCRDQAQYGLTDEVHQLEATIAALKQKLAQAQDALDALCKHLARLQADIACKANSMLLDTKCMDTRRKLTVPAERFVPEVDTFTRTTNSTLSPLKSCQLELA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 tektin 2 (testicular)
别名 H-tektin-T, TEKTB1, Tektin 2, Tektin B1, Tektin-2
计算分子量 430 aa, 50 kDa
观测分子量 54 kDa
GenBank蛋白编号BC035620
基因名称 TEKT2
Gene ID (NCBI) 27285
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9UIF3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TEKT2 belongs to the tektin family. Tektins comprise a family of filament-forming proteins that are coassembled with tubulins to form ciliary and flagellar microtubules. TEKT2 and other members of the tektin protein family form filamentous polymers in the walls of ciliary and flagellar microtubules. Western blot analysis of testis showed a TEKT2 protein with an apparent molecular mass of 54 kDa (PMID: 12029069).

实验方案

Product Specific Protocols
WB protocol for TEKT2 antibody 87634-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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