验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells |
This antibody also recognizes the phosphorylation of RPS6KA1 at Ser221.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
87534-1-RR targets Phospho-RPS6KA3 (Ser227) in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | ribosomal protein S6 kinase, 90kDa, polypeptide 3 |
| 别名 | p90RSK3 (phospho Ser227), Phospho RSK2 (Ser227), Phospho-RPS6KA3, Phospho-RSK2, Phospho-RSK2 (Ser227) |
| 计算分子量 | 740 aa, 84 kDa |
| 观测分子量 | 84 kDa |
| GenBank蛋白编号 | BC096301 |
| 基因名称 | RSK2 |
| Gene ID (NCBI) | 6197 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P51812 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Ribosomal S6 Kinase 2 (RSK2), a member of the p90RSK family of serine/threonine kinases, is known to be phosphorylated at Ser227 and Th577 in response to Adriamycin-induced DNA damage. This phosphorylation promotes RSK2 nuclear translocation and enhances RSK2 and Atm interactions in the nuclear fraction, playing an important role in the DNA damage pathway that maintains genomic stability by mediating cell cycle progression and DNA repair.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Phospho-RPS6KA3 (Ser227) antibody 87534-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
