验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, NIH/3T3 cells, RAW 264.7 cells, PC-12 cells |
| Positive IF/ICC detected in | λ phosphatase treated HeLa cells |
| Positive FC (Intra) detected in | λ phosphatase treated HeLa cells |
This antibody recognizes the phosphorylation at the Thr198 site of PKA-C (including PRKACA, PRKACB, and PRKACG).
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.13 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
产品信息
87365-3-RR targets Phospho-PKA C (Thr198) in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IF/ICC, FC (Intra), ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | mouse |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | protein kinase, cAMP-dependent, catalytic, alpha |
| 别名 | Phospho-PKA C (Thr197), Phos-PKA C, P-PKA C, PRKACA, cAMP-dependent protein kinase catalytic subunit alpha |
| 计算分子量 | 41 kDa |
| 观测分子量 | 38-43 kDa |
| GenBank蛋白编号 | NM_002730 |
| 基因名称 | PRKACA |
| Gene ID (NCBI) | 5566 |
| RRID | AB_3745487 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P17612 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Protein kinase A (PKA) is a 3′-5′-cyclic adenosine monophosphate (cAMP)-dependent kinase that is at the center of diverse biological functions in numerous systems. Inactive PKA is composed of two catalytic (PKA-C) and two regulatory (PKA-R) subunits. The binding of cAMP to the PKA-R subunits activates the enzyme, causing the release of PKA-C. Humans have three genes encoding PKA-C: PRKACA, PRKACB, and PRKACG, and two types of PKA-R's: type PKA-RI (PKA-Riα and PKA-Riβ) and type PKA-RII (PKA-RIIα and PKA-RIIβ). In a direct side-by-side assay, λ-phosphatase nearly completely removed the phosphate from Akt1 Thr308, yet dephosphorylated PKAc at activation-loop Thr197 only marginally. Shielding of this phosphate by the kinase's own N- and C-terminal regions makes Thr197 behave as a constitutive maturation mark rather than a stimulus-regulated switch. (PMID: 36431013, PMID: 39592424, PMID: 31964716)
This antibody recognizes the phosphorylation at the Thr198 site of PKA-C (including PRKACA, PRKACB, and PRKACG). After removing methionine, the site is also called Thr197.
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for Phospho-PKA C (Thr198) antibody 87365-3-RR | Download protocol |
| IF protocol for Phospho-PKA C (Thr198) antibody 87365-3-RR | Download protocol |
| WB protocol for Phospho-PKA C (Thr198) antibody 87365-3-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Phytomedicine Differential and complementary effects of Baizhu and Fuling on spleen deficiency syndrome by regulating microbiota-gut-metabolite axis. | ||
Int Immunopharmacol Melatonin orchestrates cAMP-PKA signaling to alleviate zearalenone-impaired the estradiol synthesis in mammal ovary. | ||
Purinergic Signal Targeting P2Y(14)R inhibits proliferation of cervical cancer cells and organoids and activates cAMP/PKA/CREB signaling pathway. |
