验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, HepG2 cells, U2OS cells, mouse testis tissue, rat testis tissue, mouse brain tissue, rat brain tissue |
| Positive IF/ICC detected in | Jurkat cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
87297-1-RR targets DTD1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag11510 Product name: Recombinant human DTD1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-209 aa of BC045167 Sequence: MKAVVQRVTRASVTVGGEQISAIGRGICVLLGISLEDTQKELEHMVRKILNLRVFEDESGKHWSKSVMDKQYEILCVSQFTLQCVLKGNKPDFNLAMPTEQAEGFYNSFLEQLRKTYRPELIKDGKFGAYMQVHIQNDGPVTIELESPAPGTATSDPKQLSKLEKQQQRKEKTRAKGPSESSKERNTPRKEDRSASSGAEGDVSSEREP 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | D-tyrosyl-tRNA deacylase 1 homolog (S. cerevisiae) |
| 别名 | C20orf88, D-aminoacyl-tRNA deacylase 1, DNA-unwinding element-binding protein B, DTD, DUEB |
| 计算分子量 | 209 aa, 23 kDa |
| 观测分子量 | 23 kDa |
| GenBank蛋白编号 | BC045167 |
| 基因名称 | DTD1 |
| Gene ID (NCBI) | 92675 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q8TEA8 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
DTD1 (D-aminoacyl-tRNA deacylase 1) is a crucial enzyme that ensures the fidelity of protein synthesis by maintaining L-homochirality. It functions as a proofreading factor that hydrolyzes mischarged D-aminoacyl-tRNAs into free D-amino acids and tRNA molecules, preventing the incorporation of D-amino acids into polypeptide chains. DTD1 also plays a significant part in the initiation of DNA replication by facilitating the loading of the CDC45 complex onto pre-replication sites. (PMID: 18700836;27200345)
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for DTD1 antibody 87297-1-RR | Download protocol |
| WB protocol for DTD1 antibody 87297-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
