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验证数据展示

  • WB analysis using 87291-1-RR

    WB analysis using 87291-1-RR

    Non-treated A431 cells and EGF (HZ-1326) treated A431 cells were subjected to SDS PAGE followed by western blot with 87291-1-RR (Phospho-EGFR (Thr693) antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control.

  • Schematic diagram of the phosphorylation sites of EGFR.

    Schematic diagram of the phosphorylation sites of EGFR.

    In the full-length versus signal-peptide-removed forms, nomenclature differs but the modification site is conserved.

Phospho-EGFR (Thr693) Recombinant monoclonal antibody

Phospho-EGFR (Thr693) Uni-rAb® Recombinant Antibody for WB, ELISA
Cat No. 87291-1-RR

产品说明书

CloneNo. 252344B6

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

EGF Receptor (Thr669), p-EGFR (T669), p-EGFR (T693), p-EGFR (Thr669), p-EGFR (Thr693)

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inEGF treated A431 cells

This antibody, also known as EGFR Thr669, is the site where the signal peptide is removed.

Planning an IHC experiment? We recommend our IHCeasy EGFR Ready-To-Use IHC Kit. EGFR primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

87291-1-RR targets Phospho-EGFR (Thr693) in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian)
别名 EGF Receptor (Thr669), p-EGFR (T669), p-EGFR (T693), p-EGFR (Thr669), p-EGFR (Thr693)
观测分子量150-180 kDa
GenBank蛋白编号NM_005228
基因名称 EGFR
Gene ID (NCBI) 1956
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP00533
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Epidermal growth factor receptor (EGFR) is a member of the receptor tyrosine kinase (RTK) family and plays a critical role in a wide variety of cellular functions, including proliferation, differentiation and apoptosis. Phospho-EGFR (Thr693) is a phosphorylated form of the epidermal growth factor receptor (EGFR) at the threonine 693 residue. This phosphorylation event is significant as it is involved in the activation of EGFR signaling pathways. EGFR is known to be a transcriptional co-activator for several cancer-promoting genes, including cyclin D1, nitric oxide synthase (iNOS), and cyclooxygenase-2 (COX-2). (PMID: 34295309) Some scientists call EGFR Thr693 as EGFR Thr669, the site where the signaling peptide is removed.

实验方案

Product Specific Protocols
WB protocol for Phospho-EGFR (Thr693) antibody 87291-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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