验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, MCF-7 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
87281-1-RR targets PLAGL1 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag23073 Product name: Recombinant human PLAGL1 protein Source: e coli.-derived, PET30a Tag: 6*His Domain: 201-463 aa of BC074814 Sequence: RHTKKTHSQELMKESLQTGDLLSTFHTISPSFQLKAAALPPFPLGASAQNGLASSLPAEVHSLTLSPPEQAAQPMQPLPESLASLHPSVSPGSPPPPLPNHKYNTTSTSYSPLASLPLKADTKGFCNISLFEDLPLQEPQSPQKLNPGFDLAKGNAGKVNLPKELPADAVNLTIPASLDLSPLLGFWQLPPPATQNTFGNSTLALGPGESLPHRLSCLGQQQQEPPLAMGTVSLGQLPLPPIPHVFSAGTGSAILPHFHHAFR 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | pleiomorphic adenoma gene-like 1 |
| 别名 | Lost on transformation 1, LOT 1, LOT1, LOT-1, Pleiomorphic adenoma-like protein 1 |
| 计算分子量 | 463 aa, 51 kDa |
| 观测分子量 | 51 kDa |
| GenBank蛋白编号 | BC074814 |
| 基因名称 | PLAGL1 |
| Gene ID (NCBI) | 5325 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q9UM63 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PLAGL1 (Pleomorphic adenoma gene-like 1) is a crucial zinc-finger transcription factor acting as a tumor suppressor, regulating cell cycle, growth (apoptosis), and embryonic development, particularly involved in pancreatic function and imprinted expression (paternal allele). While its family member PLAG1 is an oncogene in salivary tumors, PLAGL1 often suppresses tumors, though its dysregulation is linked to transient neonatal diabetes mellitus (TNDM) and certain cancers, highlighting its complex role in growth control and metabolic homeostasis.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PLAGL1 antibody 87281-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
