验证数据展示
经过测试的应用
| Positive WB detected in | EGF treated A431 cells, Serum-free treated and EGF treated A431 cells |
| Positive IF/ICC detected in | EGF treated A431 cells |
This antibody, also known as EGFR Tyr1148, is the site where the signal peptide is removed.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
87164-1-RR targets Phospho-EGFR (Tyr1172) in WB, IF/ICC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian) |
| 别名 | EGFR (phospho Y1172), EGFR (Tyr1172), p-EGF Receptor, p-EGF Receptor (Tyr1148), p-EGFR |
| 计算分子量 | 1210 aa, 134 kDa |
| 观测分子量 | 175 kDa |
| GenBank蛋白编号 | BC094761 |
| 基因名称 | EGFR |
| Gene ID (NCBI) | 1956 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P00533 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ERBB1, also named as EGFR, is a receptor for EGF, but also for other members of the EGF family, as TGF-alpha, amphiregulin, betacellulin, heparin-binding EGF-like growth factor, GP30 and vaccinia virus growth factor. Phospho-EGFR (Tyr1172) is a phosphorylated form of the epidermal growth factor receptor (EGFR), which is a transmembrane glycoprotein receptor that belongs to the ErbB family of receptor tyrosine kinases (RTKs). This phosphorylation event is significant as it activates downstream signaling cascades that regulate various cellular processes, including cell growth, proliferation, and survival. Some scientists call EGFR Tyr1172 as EGFR Tyr1148, the site where the signaling peptide is removed.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for Phospho-EGFR (Tyr1172) antibody 87164-1-RR | Download protocol |
| WB protocol for Phospho-EGFR (Tyr1172) antibody 87164-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
