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验证数据展示

  • WB analysis using 87145-2-RR

    WB analysis using 87145-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 87145-2-RR (CD1C antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human tonsillitis using 87145-2-RR

    IHC staining of human tonsillitis using 87145-2-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 87145-2-RR (CD1c antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of Jurkat using 87145-2-RR

    IF Staining of Jurkat using 87145-2-RR

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using CD1C antibody (87145-2-RR, Clone: 252399G7 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity of 87145-2-RR

    Affinity of 87145-2-RR

    Biolayer interferometry (BLl) kinetic assays of 87145-2-RR against Human CD1c were performed. The affinity constant is 15.7 nM.

CD1c Recombinant monoclonal antibody

CD1c Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 87145-2-RR

产品说明书

CloneNo. 252399G7

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

BDCA1, CD1, CD1c molecule, R7, T-cell surface glycoprotein CD1c

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inJurkat cells, MOLT-4 cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inJurkat cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

87145-2-RR targets CD1c in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Eg9187

Product name: Recombinant human B2M-CD1C protein

Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

Tag: C-Twin-Strep-Tag

Domain: 18-333 aa of

Sequence: NADASQEHVSFHVIQIFSFVNQSWARGQGSGWLDELQTHGWDSESGTIIFLHNWSKGNFSNEELSDLELLFRFYLFGLTREIQDHASQDYSKYPFEVQVKAGCELHSGKSPEGFFQVAFNGLDLLSFQNTTWVPSPGCGSLAQSVCHLLNHQYEGVTETVYNLIRSTCPRFLLGLLDAGKMYVHRQVRPEAWLSSRPSLGSGQLLLVCHASGFYPKPVWVTWMRNEQEQLGTKHGDILPNADGTWYLQVILEVASEEPAGLSCRVRHSSLGGQDIILYWGHHFSM

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 CD1c molecule
别名 BDCA1, CD1, CD1c molecule, R7, T-cell surface glycoprotein CD1c
观测分子量38 kDa
基因名称 CD1C
Gene ID (NCBI) 911
RRIDAB_3745368
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP29017
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD1c molecule (CD1c, also known as R7, CD1, CD1A, and BDCA1) is a member of the CD1 family of transmembrane glycoproteins, which is synthesized in the endoplasmic reticulum and expressed on the monocytes, marginal zone B cells in lymph nodes as well as peripheral blood (PMID: 23468110). The CD1 proteins belong to the family of major histocompatibility complex (MHC) class I-like proteins that present lipid-based antigens to T cells (PMID: 15032598; 23127489). CD1c shows interaction with both αβ or γδ T cells and mediates T cell responses against mycobacterium tuberculosis (PMID: 10727456; 10786796).

实验方案

Product Specific Protocols
IF protocol for CD1c antibody 87145-2-RRDownload protocol
IHC protocol for CD1c antibody 87145-2-RRDownload protocol
WB protocol for CD1c antibody 87145-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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