验证数据展示
经过测试的应用
| Positive WB detected in | SW480 cells, K-562 cells, HEK-293 cells, HepG2 cells, PANC-1 cells |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
87117-4-RR targets MINPP1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag34998 Product name: Recombinant human MINPP1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-339 aa of BC032504 Sequence: MLRAPGCLLRTSVAPAAALAAALLSSLARCSLLEPRDPVASSLSPYFGTKTRYEDVNPVLLSGPEAPWRDPELLEGTCTPVQLVALIRHGTRYPTVKQIRKLRQLHGLLQARGSRDGGASSTGSRDLGAALADWPLWYADWMDGQLVEKGRQDMRQLALRLASLFPALFSRENYGRLRLITSSKHRCMDSSAAFLQGLWQHYHPGLPPPDVADMEFGPPTVNDKLMRFFDHCEKFLTEVEKNATALYHVEAFKTGPEMQNILKKVAATLQVPVNDLNADLIQVAFFTCSFDLAIKGVKSPWCDVFDIDDAKVLEYLNDLKQYWKRGYGYTINSRSSCTL 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | multiple inositol polyphosphate histidine phosphatase, 1 |
| 别名 | HIPER1, Ins(1,3,4,5)P(4) 3 phosphatase, MINPP2, MIPP |
| 计算分子量 | 55 kDa |
| 观测分子量 | 45-50 kDa |
| GenBank蛋白编号 | BC032504 |
| 基因名称 | MINPP1 |
| Gene ID (NCBI) | 9562 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q9UNW1 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MINPP1 (multiple inositol-polyphosphate phosphatase 1), also known as MIPP and MINPP2, is an enzyme that catalyzes the removal of the 3-phosphate group from inositol phosphate substrates. It plays a crucial role in the metabolism of inositol polyphosphates, which are important second messengers in cellular signaling pathways. MINPP1 has been implicated in various diseases, including certain types of cancer, where its expression levels may be associated with tumor progression and differentiation.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for MINPP1 antibody 87117-4-RR | Download protocol |
| WB protocol for MINPP1 antibody 87117-4-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
