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验证数据展示

  • WB analysis using 87100-1-RR

    WB analysis using 87100-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 87100-1-RR (Apolipoprotein CIII antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis using 87100-1-RR

    WB analysis using 87100-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 87100-1-RR (Apolipoprotein CIII antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of L02 using 87100-1-RR

    IF Staining of L02 using 87100-1-RR

    Immunofluorescent analysis of (4% PFA) fixed L02 cells using Apolipoprotein CIII antibody (87100-1-RR, Clone: 252153H5 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 87100-1-RR

    Affinity and Kinetic Characterization of 87100-1-RR

    Biolayer interferometry (BLl) kinetic assays of 87100-1-RR against Human Apolipoprotein CIII were performed. The affinity constant is below 1 pM.

Apolipoprotein CIII Recombinant monoclonal antibody

Apolipoprotein CIII Uni-rAb® Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 87100-1-RR

产品说明书

CloneNo. 252153H5

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

APOC3, Apo CIII, ApoC III, APOCIII, Apo-CIII

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inhuman plasma, human serum
Positive IF/ICC detected inL02 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

87100-1-RR targets Apolipoprotein CIII in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Eg3387

Product name: recombinant human APOC3 protein

Source: mammalian cells-derived, pHZ-KIsec-N-rFc

Tag: N-rFc

Domain: 21-99 aa of NM_000040

Sequence: SEAEDASLLSFMQGYMKHATKTAKDALSSVQESQVAQQARGWVTDGFSSLKDYWSTVKDKFSEFWDLDPEVRPTSAVAA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 apolipoprotein C-III
别名 APOC3, Apo CIII, ApoC III, APOCIII, Apo-CIII
计算分子量 11 kDa
观测分子量11 kDa
GenBank蛋白编号NM_000040
基因名称 Apolipoprotein CIII
Gene ID (NCBI) 345
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP02656
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Apolipoprotein CIII (apoCIII) is the most abundant C-apolipoprotein in human and is found on very low density lipoproteins, chylomicrons, and high density lipoproteins (HDL) (PMID: 18408013). ApoCIII inhibits lipoprotein lipase as well as binding of lipoproteins to cell surface heparan sulfate proteoglycans and receptors (PMID: 26228667). ApoC-III has 3 actions that can impair plasma lipoprotein metabolism and lead to hypertriglyceridemia (PMID: 24941081).

实验方案

Product Specific Protocols
IF protocol for Apolipoprotein CIII antibody 87100-1-RRDownload protocol
WB protocol for Apolipoprotein CIII antibody 87100-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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