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验证数据展示

  • WB analysis of mouse brain using 87053-2-RR

    WB analysis of mouse brain using 87053-2-RR

    Non-treated mouse brain tissue, phosphatase inhibitor treated mouse brain tissue and λ phosphatase treated mouse brain tissue were subjected to SDS PAGE followed by western blot with 87053-2-RR (Phospho-TAU (Ser199/202) antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control.

  • WB analysis of SH-SY5Y using 87053-2-RR

    WB analysis of SH-SY5Y using 87053-2-RR

    Non-treated and Calyculin A treated SH-SY5Y cellls were subjected to SDS PAGE followed by western blot with 87053-2-RR (Phospho-TAU (Ser199/202) antibody) at dilution of 1:20000 incubated at room temperature for 1 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control.

Phospho-TAU (Ser199/202) Recombinant monoclonal antibody

Phospho-TAU (Ser199/202) Uni-rAb® Recombinant Antibody for WB, ELISA
Cat No. 87053-2-RR

产品说明书

CloneNo. 252389B6

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, ELISA

别名

P TAU (Ser199/202), phos TAU, Phos TAU (Ser199/202), Phospho TAU (Ser199/202), Phospho TAU Ser199/202

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse brain tissue, Calyculin A treated SH-SY5Y cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

87053-2-RR targets Phospho-TAU (Ser199/202) in WB, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 microtubule-associated protein tau
别名 P TAU (Ser199/202), phos TAU, Phos TAU (Ser199/202), Phospho TAU (Ser199/202), Phospho TAU Ser199/202
计算分子量 37-46, 79-81 kDa
观测分子量45-60 kDa
GenBank蛋白编号BC000558
基因名称 TAU
Gene ID (NCBI) 4137
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP10636
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Tau is hyperphosphorylated during aging and in age-related neurodegenerative diseases such as Alzheimer's disease (AD) and fronto-temporal dementia. Hyperphosphorylation of Tau leads to the formation of neurofibrillary tangles (NFT) in the neurons and glia cells, which is one of the hallmarks of AD.

实验方案

Product Specific Protocols
WB protocol for Phospho-TAU (Ser199/202) antibody 87053-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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