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验证数据展示

  • WB analysis using 87037-1-RR (same clone as 87037-1-PBS)

    WB analysis using 87037-1-RR (same clone as 87037-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 87037-1-RR (P5CS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87037-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 87037-1-RR (same clone as 87037-1-PBS)

    IF Staining of HeLa using 87037-1-RR (same clone as 87037-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using P5CS antibody (87037-1-RR, Clone: 252125B12 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 87037-1-PBS in a different storage buffer formulation.

  • IHC staining of rat brain using 87037-1-RR (same clone as 87037-1-PBS)

    IHC staining of rat brain using 87037-1-RR (same clone as 87037-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 87037-1-RR (P5CS antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 87037-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA sample test of MP02872-1

    Sandwich ELISA sample test of MP02872-1

    The mean P5CS concentration was determined to be 749.95 ng/mL in HeLa cell extract based on a 1.2 mg/mL extract load and 1,046.66 ng/mL in MCF-7 cell extract based on a 1.2 mg/mL extract load.

  • Sandwich ELISA standard curve of MP02872-1

    Sandwich ELISA standard curve of MP02872-1

    Sandwich ELISA standard curve of MP02872-1, Human GNAS Recombinant Matched Antibody Pair - PBS only. 87037-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag12100. 87037-1-PBS was HRP conjugated as the detection antibody. Range: 7.81-500 ng/mL

P5CS Recombinant monoclonal antibody, PBS Only (Detector)

P5CS Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, Sandwich ELISA, Indirect ELISA
Cat No. 87037-1-PBS

产品说明书

CloneNo. 252125B12

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Sandwich ELISA, Indirect ELISA

别名

ALDH18A1, Aldehyde dehydrogenase family 18 member A1, Delta-1-pyrroline-5-carboxylate synthase, Gamma-glutamyl kinase, Gamma-glutamyl phosphate reductase

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

87037-1-PBS targets P5CS as part of a matched antibody pair:

MP02872-1: 87037-2-PBS capture and 87037-1-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, IF/ICC, Sandwich ELISA, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag12100

Product name: Recombinant human ALDH18A1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-362 aa of BC106054

Sequence: MLSQVYRCGFQPFNQHLLPWVKCTTVFRSHCIQPSVIRHVRSWSNIPFITVPLSRTHGKSFAHRSELKHAKRIVVKLGSAVVTRGDECGLALGRLASIVEQVSVLQNQGREMMLVTSGAVAFGKQRLRHEILLSQSVRQALHSGQNQLKEMAIPVLEARACAAAGQSGLMALYEAMFTQYSICAAQILVTNLDFHDEQKRRNLNGTLHELLRMNIVPIVNTNDAVVPPAEPNSDLQGVNVISVKDNDSLAARLAVEMKTDLLIVLSDVEGLFDSPPGSDDAKLIDIFYPGDQQSVTFGIKSRVGMGGMEAKVKAALWALQGGTSVVIANGTHPKVSGHVITDIVEGKKVGTFFSEVKPAGPT

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 aldehyde dehydrogenase 18 family, member A1
别名 ALDH18A1, Aldehyde dehydrogenase family 18 member A1, Delta-1-pyrroline-5-carboxylate synthase, Gamma-glutamyl kinase, Gamma-glutamyl phosphate reductase
计算分子量 795 aa, 87 kDa
观测分子量75-87 kDa
GenBank蛋白编号BC106054
基因名称 P5CS
Gene ID (NCBI) 5832
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP54886
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Delta 1-pyrroline-5-carboxylate synthetase (P5CS), also named as GSAS and PYCS, catalyzes the ATP and the NAD(P)H-dependent conversion of L-glutamate to glutamic gamma-semialdehyde (GSA) which is the metabolic precursor for proline biosynthesis(PMID:8761662). This protein includes 2 domains: glutamate 5-kinase and gamma-glutamyl phosphate reductase. In the N-terminal section, it belongs to the glutamate 5-kinase family and in the C-terminal section, it belongs to the gamma-glutamyl phosphate reductase family. Defects in ALDH18A1 are the cause of cutis laxa, autosomal recessive, type 3A (ARCL3A)(PMID:18478038). It has 2 isoforms produced by alternative splicing.

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